caa a22 4500 465767451 CHVBK 20180323111918.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00294215 doi (NATIONALLICENCE)springer-10.1007/BF00294215 Nag S. Department of Pathology, Queen's University and Kingston General Hospital, K7L 3N6, Kingston, Ontario, Canada aut Ultracytochemical localisation of Na+, K+-ATPase in cerebral endothelium in acute hypertension [Elektronische Daten] [S. Nag] Summary: This ultracytochemical study was undertaken to determine whether increased arteriolar permeability in acute hypertension is accompanied by altered localisation of the ouabain-sensitive, K+-dependent p-nitrophenylphosphatase (K+-NPPase), a component of the Na+, K+-ATPase system. Rats were injected with horseradish peroxidase (HRP) intravenously and acute hypertension was induced by a 2-min infusion of angiotensin amide. Rats were killed at 3 and 15 min, following which brains were sliced and reacted for demonstration of K+-NPPase and HRP reaction product. Vessels of normotensive and hypertensive rats that were nonpermeable to HRP showed discontinuous distribution of K+-NPPase on the outer plasma membranes of endothelial and adventitial cells of arterioles and endothelial cells and pericytes of capillaries. Arterioles of the hypertensive rats which were permcable to HRP showed marked reduction of K+-NPPase localisation in their walls at 3 min while at 15 min when the blood pressure had returned to resting levels the enzyme localisation was similar to controls. This study demonstrates transient alteration of the NA+, K+-ATPase system during increased endothelial permeability in acute hypertension. The implication of this finding and our previous observation of reduced Ca2+-ATPase localisation in endothelial plasma membranes in acute hypertension has been discussed. Springer-Verlag, 1990 Na+, K+ ATPase nationallicence Blood-brain barrier nationallicence Hypertension nationallicence Cerebral vessels nationallicence Acta Neuropathologica Springer-Verlag 80/1(1990-05-01), 7-11 0001-6322 80:1<7 1990 80 401 https://doi.org/10.1007/BF00294215 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00294215 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Nag S. Department of Pathology, Queen's University and Kingston General Hospital, K7L 3N6, Kingston, Ontario, Canada aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 80/1(1990-05-01), 7-11 0001-6322 80:1<7 1990 80 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer