caa a22 4500 465767478 CHVBK 20180323111918.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00294216 doi (NATIONALLICENCE)springer-10.1007/BF00294216 Increased 5-hydroxytryptamine immunoreactivity in traumatized spinal cord [Elektronische Daten] An experimental study in the rat [H. Sharma, J. Westman, Y. Olsson, O. Johansson, P. Dey] Summary: The possibility that serotonin (5-hydroxytryptamine, 5-HT) is involved in the early tissue reactions occurring in spinal cord trauma was examined in a rat model using an immunocytochemical technique. The injury was made in the form of a 5-mm long and 2.5-mm wide lesion of the right dorsal horn at the level of T10-11. Injured rats, pretreated with the 5-HT synthesis blocking agent, p-chlorophenyl alanine (p-CPA) were compared with untreated injured controls and the animals were allowed to survive for 5 h. The distribution of 5-HT was examined in proximal and distal cross-sections of the cord, located 2 and 5 mm away from the injury. Normal rats showed immunoreactive material in nerve cell processes and in a few nerve cell bodies of the ventral horns. The trauma to the spinal cord caused a marked increase in 5-HT immunoreactivity in the segments located 2 mm proximal and distal to the injury, particularly in the ipsilateral ventral horn. The segment located 5 mm distal to the lesion showed a similar increase in immunoreactivity but it was apparently less pronounced in the corresponding proximal segment. Treatment with p-CPA markedly reduced the trauma-induced increase in 5-HT immunoreactivity in all the segments. These immunohistochemical findings were in line with the changes in the contents of 5-HT measured biochemically in corresponding spinal cord segments. At the onset of the trauma to the spinal cord 5-HT is thus present in the tissue, mainly in the form of 5-HT-containing nerve cell processes. Biochemical determinations also revealed that there is an increased amount of 5-HT in the traumatized spinal cord. The present study indicates that this is at least partly due to an increased amount of 5-HT in neurons and nerve cell processes of the perifocal region. The pathophysiollogical significance of the observed 5-HT-reaction in spinal cord injury is not known in all its details. However, 5-HT might be implicated in such tissue reaction, such as increased microvascular permea bility and edema formation occurring in the early period after trauma. Springer-Verlag, 1990 Serotonin (5-hydroxytryptamine, 5-HT) nationallicence Spinal cord trauma nationallicence Ventral horn nationallicence p -Chlorophenyl alanine nationallicence Immunohistochemistry nationallicence Sharma H. Laboratory of Neuropathology, Institute of Pathology, University Hospital, S-75185, Uppsala, Sweden aut Westman J. Department of Human Anatomy, Uppsala University, Uppsala, Sweden aut Olsson Y. Laboratory of Neuropathology, Institute of Pathology, University Hospital, S-75185, Uppsala, Sweden aut Johansson O. Department of Histology, Karolinska Institute, Stockholm, Sweden aut Dey P. Neurophysiology Research Unit, Department of Physiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India aut Acta Neuropathologica Springer-Verlag 80/1(1990-05-01), 12-17 0001-6322 80:1<12 1990 80 401 https://doi.org/10.1007/BF00294216 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00294216 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sharma H. Laboratory of Neuropathology, Institute of Pathology, University Hospital, S-75185, Uppsala, Sweden aut NATIONALLICENCE 700 1- Westman J. Department of Human Anatomy, Uppsala University, Uppsala, Sweden aut NATIONALLICENCE 700 1- Olsson Y. Laboratory of Neuropathology, Institute of Pathology, University Hospital, S-75185, Uppsala, Sweden aut NATIONALLICENCE 700 1- Johansson O. Department of Histology, Karolinska Institute, Stockholm, Sweden aut NATIONALLICENCE 700 1- Dey P. Neurophysiology Research Unit, Department of Physiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 80/1(1990-05-01), 12-17 0001-6322 80:1<12 1990 80 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer