caa a22 4500 465767494 CHVBK 20180323111918.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00294214 doi (NATIONALLICENCE)springer-10.1007/BF00294214 The mRNA encoding the scrapie agent protein is present in a variety of non-neuronal cells [Elektronische Daten] [H. Brown, N. Goller, R. Rudelli, G. Merz, G. Wolfe, H. Wisniewski, N. Robakis] Summary: PrP 27-30, a unique protease-resistant protein associated with scrapie infectivity, derives from the proteolytic cleavage of a larger precursor encoded by a host gene. To identify sites of PrP biosynthesis, in situ hybridization was done using cloned PrP cDNA as a probe. In rodent brain, PrP mRNA was expressed in neurons, ependymal cells, choroid plexus epithelium, astrocytes, pericytes, endothelial cells and meninges of both scrapie-infected and uninfected animals. PrP mRNA was also detected in vitro in isolated brain microglia cells. Pulmonary cells and heart muscle cells contained high levels of this mRNA. Hybridization was not detected in spleen, confirming earlier RNA blot experiments indicating extremely low levels of PrP mRNA in this tissue. Results indicate that PrP mRNA is a normal component in a variety of non-neuronal tissues and may explain the origin of the amyloid plaques present in the subependymal region of scrapie-infected brain. Springer-Verlag, 1990 Scrapie nationallicence In situ hybridization nationallicence Unconventional agents nationallicence Protease-resistant protein nationallicence Brown H. New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, 10314, Staten Island, NY, USA aut Goller N. New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, 10314, Staten Island, NY, USA aut Rudelli R. New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, 10314, Staten Island, NY, USA aut Merz G. New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, 10314, Staten Island, NY, USA aut Wolfe G. New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, 10314, Staten Island, NY, USA aut Wisniewski H. New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, 10314, Staten Island, NY, USA aut Robakis N. New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, 10314, Staten Island, NY, USA aut Acta Neuropathologica Springer-Verlag 80/1(1990-05-01), 1-6 0001-6322 80:1<1 1990 80 401 https://doi.org/10.1007/BF00294214 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00294214 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Brown H. New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, 10314, Staten Island, NY, USA aut NATIONALLICENCE 700 1- Goller N. New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, 10314, Staten Island, NY, USA aut NATIONALLICENCE 700 1- Rudelli R. New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, 10314, Staten Island, NY, USA aut NATIONALLICENCE 700 1- Merz G. New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, 10314, Staten Island, NY, USA aut NATIONALLICENCE 700 1- Wolfe G. New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, 10314, Staten Island, NY, USA aut NATIONALLICENCE 700 1- Wisniewski H. New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, 10314, Staten Island, NY, USA aut NATIONALLICENCE 700 1- Robakis N. New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, 10314, Staten Island, NY, USA aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 80/1(1990-05-01), 1-6 0001-6322 80:1<1 1990 80 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer