caa a22 4500 465767621 CHVBK 20180323111919.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00294222 doi (NATIONALLICENCE)springer-10.1007/BF00294222 Ultrastructural and biochemical findings in brain cell cultures infected with canine distemper virus [Elektronische Daten] [T. Glaus, C. Griot, A. Richard, U. Althaus, N. Herschkowitz, M. Vandevelde] Summary: To study the pathomechanism of demyelination in canine distemper (CD), dog brain cell cultures were infected with virulent A75/17-CD virus (CDV) and examined ultrastructurally. Special attention was paid to the oligodendrocytes, which were specifically immunolabelled. In addition, cerebroside sulfotransferase (CST), an enzyme specific for oligodendrocyte activity was assayed during the course of the infection. Infection and maturation as well as CDV-induced changes were found in astrocytes and brain macrophages. Infection of oligodendrocytes was rarely seen, although CST activity of the culture markedly decreased and vacuolar degeneration of these cells occurred, resulting in their complete disappearance. We concluded that the degeneration of oligodendrocytes and demyelination is not due to direct virus-oligodendrocyte interaction, but due to CDV-induced events in other glial cells. Springer-Verlag, 1990 Brain cell culture nationallicence Canine distemper virus nationallicence Cerebroside sulfotransferase nationallicence Electron microscopy nationallicence Oligodendrocytes nationallicence Glaus T. Institute of Animal Neurology, University of Berne, P. O. Box 2735, CH-3001, Berne, Switzerland aut Griot C. Institute of Animal Neurology, University of Berne, P. O. Box 2735, CH-3001, Berne, Switzerland aut Richard A. Institute of Animal Neurology, University of Berne, P. O. Box 2735, CH-3001, Berne, Switzerland aut Althaus U. Department of Pediatrics, School of Medicine, University of Berne, CH-3011, Berne, Switzerland aut Herschkowitz N. Department of Pediatrics, School of Medicine, University of Berne, CH-3011, Berne, Switzerland aut Vandevelde M. Institute of Animal Neurology, University of Berne, P. O. Box 2735, CH-3001, Berne, Switzerland aut Acta Neuropathologica Springer-Verlag 80/1(1990-05-01), 59-67 0001-6322 80:1<59 1990 80 401 https://doi.org/10.1007/BF00294222 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00294222 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Glaus T. Institute of Animal Neurology, University of Berne, P. O. Box 2735, CH-3001, Berne, Switzerland aut NATIONALLICENCE 700 1- Griot C. Institute of Animal Neurology, University of Berne, P. O. Box 2735, CH-3001, Berne, Switzerland aut NATIONALLICENCE 700 1- Richard A. Institute of Animal Neurology, University of Berne, P. O. Box 2735, CH-3001, Berne, Switzerland aut NATIONALLICENCE 700 1- Althaus U. Department of Pediatrics, School of Medicine, University of Berne, CH-3011, Berne, Switzerland aut NATIONALLICENCE 700 1- Herschkowitz N. Department of Pediatrics, School of Medicine, University of Berne, CH-3011, Berne, Switzerland aut NATIONALLICENCE 700 1- Vandevelde M. Institute of Animal Neurology, University of Berne, P. O. Box 2735, CH-3001, Berne, Switzerland aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 80/1(1990-05-01), 59-67 0001-6322 80:1<59 1990 80 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer