caa a22 4500 465767702 CHVBK 20180323111919.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00307629 doi (NATIONALLICENCE)springer-10.1007/BF00307629 Observations on the muscle plasma membrane-associated cytoskeletons of mdx mice by quick-freeze, deep-etch, rotary-shadow replica method [Elektronische Daten] [Y. Wakayama, S. Shibuya] Summary: The Duchenne muscular dystrophy gene product "dystrophin” is a sarcolemma-associated cytoskeleton which is present just inside the sarcolemma. We investigated the ultrastructure and the relationship of this cytoskeleton to the muscle plasma membrane by immunoelectron microscopy and freeze-etch electron microscopy of liquid helium-frozen fresh muscles. The immunoelectron microscopy of the extensor digitorum longus muscles of six mdx mice and six control mice showed the location of anti-dystrophin antibody along the muscle plasma membrane undercoat of all the muscle samples from the control mice without any antibody reaction in the mdx mice muscles. Fresh extensor digitorum longus muscles of seven mdx and six control mice were quick-frozen in liquid helium in the rapid-freeze device. High-magnification electron microscopy of the deep-etch, rotary-shadow replicas of the frozen muscles showed the network formation and attachment of individual rod-shaped cytoskeletons of variable size to the cytoplasmic surface of the muscle plasma membrane in both mdx mice and control mice. The length of cytoskeletons attached to the muscle plasma membranes was measured and mean length±SE in mdx mice and control mice were 98±4 nm and 101±3 nm, respectively. Although these values were not statistically different (P>0.1), the distribution frequency of 130-150 nm muscle plasma membrane-associated cytoskeletons was 7.9% in mdx mice versus 14.5% in control mice. Since the predicted length of dystrophin is 125-150 nm, the 130- to 150-nm plasma membrane-associated cytoskeletons of mdx control mice may include dystrophin. Springer-Verlag, 1990 mdx and control mice muscles nationallicence Dystrophin nationallicence Quick-freeze, deep-etch, rotary-shadow replica nationallicence Muscle plasma membrane-associated cytoskeletons nationallicence Wakayama Y. Division of Neurology, Department of Medicine, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Midori-ku, 227, Yokohama, Japan aut Shibuya S. Division of Neurology, Department of Medicine, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Midori-ku, 227, Yokohama, Japan aut Acta Neuropathologica Springer-Verlag 80/6(1990-10-01), 618-623 0001-6322 80:6<618 1990 80 401 https://doi.org/10.1007/BF00307629 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00307629 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wakayama Y. Division of Neurology, Department of Medicine, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Midori-ku, 227, Yokohama, Japan aut NATIONALLICENCE 700 1- Shibuya S. Division of Neurology, Department of Medicine, Showa University Fujigaoka Hospital, 1-30 Fujigaoka, Midori-ku, 227, Yokohama, Japan aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 80/6(1990-10-01), 618-623 0001-6322 80:6<618 1990 80 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer