caa a22 4500 465767893 CHVBK 20180323111920.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00294643 doi (NATIONALLICENCE)springer-10.1007/BF00294643 Immunohistological study of senile brains by using a monoclonal antibody recognizing β amyloid precursor protein: significance of granular deposits in relation with senile plaques [Elektronische Daten] [H. Takahashi, C. Kurashima, M. Utsuyama, K. Hirokawa] Summary: Immunochemical analyses revealed that a monclonal antibody Am-3 recognized β amyloid precursor protein (βAPP) in senile plaques extracted from Alzheimer's brain, but did not recognize β amyloid protein. Immunohistochemically, however, the staining pattern of Am-3 in frozen section of Alzheimer's brain was almost the same with that of rabbit polyclonal antibody to β amyloid peptide which could recognize both β amyloid protein and βAPP. In other words, βAPP was present in senile plaques of various types, cerebrovascular amyloid and granular deposits. The granular deposits were 5-10 μm in size and laminarily distributed in the 1st, 3rd and 4th layers of cerebral cortex. They were especially abundant in 1st and 4th layers where senile plaques were usually fewer in number. Although the distribution in the cerebral cortex was different between the senile plaques and the granular deposits, the number of the granular deposits was well correlated with that of senile plaques. The granular deposits were negative in Congo-red birefringence, but contained β amyloid protein as well as βAPP fragment judging from positive staining by both Am-3 and polyclonal antibody to synthetic β amyloid peptide. Thus, they could be regarded as "pre-amyloid”. Springer-Verlag, 1990 Monoclonal antibody nationallicence β Amyloid protein nationallicence β Amyloid precursor protein nationallicence Senile plaque nationallicence Granular deposits nationallicence Takahashi H. Department of Neurology, Tokyo Medical and Dental University, 35-2, Sakaecho, Itabashiku, Tokyo, Japan aut Kurashima C. Department of Pathology, Tokyo Metropolitan Institute of Gerontology, 35-2, Sakaecho, Itabashiku, Tokyo, Japan aut Utsuyama M. Department of Pathology, Tokyo Metropolitan Institute of Gerontology, 35-2, Sakaecho, Itabashiku, Tokyo, Japan aut Hirokawa K. Department of Pathology, Tokyo Metropolitan Institute of Gerontology, 35-2, Sakaecho, Itabashiku, Tokyo, Japan aut Acta Neuropathologica Springer-Verlag 80/3(1990-07-01), 260-265 0001-6322 80:3<260 1990 80 401 https://doi.org/10.1007/BF00294643 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00294643 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takahashi H. Department of Neurology, Tokyo Medical and Dental University, 35-2, Sakaecho, Itabashiku, Tokyo, Japan aut NATIONALLICENCE 700 1- Kurashima C. Department of Pathology, Tokyo Metropolitan Institute of Gerontology, 35-2, Sakaecho, Itabashiku, Tokyo, Japan aut NATIONALLICENCE 700 1- Utsuyama M. Department of Pathology, Tokyo Metropolitan Institute of Gerontology, 35-2, Sakaecho, Itabashiku, Tokyo, Japan aut NATIONALLICENCE 700 1- Hirokawa K. Department of Pathology, Tokyo Metropolitan Institute of Gerontology, 35-2, Sakaecho, Itabashiku, Tokyo, Japan aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 80/3(1990-07-01), 260-265 0001-6322 80:3<260 1990 80 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer