caa a22 4500 465767966 CHVBK 20180323111920.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00294644 doi (NATIONALLICENCE)springer-10.1007/BF00294644 Ludwin S. Department of Pathology, Kingston General Hospital, K7L 3N6, Kingston, Ontario, Canada aut Phagocytosis in the rat optic nerve following Wallerian degeneration [Elektronische Daten] [S. Ludwin] Summary: Unilateral enucleation of the eye in adult male rats was performed in an attempt to resolve the long-standing controversy as to the nature of the phagocytic cells during Wallerian degeneration in the central nervous system. Previously both resident microglia and circulating monocytes, as well as oligodendrocytes, have all been considered to be the phagocytic cells. In these present experiments macrophages and microglia were studied using lectin histochemistry for Griffonia simplicifolia agglutinin and the monoclonal antibody ED1 at light microscopic level. Oligodendrocytes were demonstrated ultrastructurally using immunohistochemistry with monoclonal antibodies against myelin oligodendrocyte glycoprotein (MOG). Ultrastructural examination of the degeneration optic nerves confirmed longstanding reports of the slow nature of breakdown in the adult central nervous system. During the early periods of breakdown, starting at 1 week and continuing to 1 or 2 months, it was difficult to type, on ultrastructural examination alone, the nature of all the cells undergoing phagocytosis, but many of them resembled microglia/macrophages. Myelin debris cleared very slowly and could still be recognised prominently in the nerve up to 22 months post-enucleation. Lectin and immunochemical examination showed that the early major phagocytic component of phagocytosis was carried out by macrophages, probably both circulating and resident. In addition, however, myelin and axonal debris was taken up or retracted into oligodendrocyte processes, which were stained with antibodies to MOG. This oligodendrocyte component appeared to be small in relationship to the overall degree of debris. Phagocytosis in the optic nerve, therefore, is in many respects similar to that in the peripheral nervous system, which has been shown to be carried out mainly by circulating macrophages, with only a minor role played by the Schwann cell. Springer-Verlag, 1990 Oligodendrocytes nationallicence Macrophages nationallicence Microglia nationallicence Wallerian degeneration nationallicence Acta Neuropathologica Springer-Verlag 80/3(1990-07-01), 266-273 0001-6322 80:3<266 1990 80 401 https://doi.org/10.1007/BF00294644 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00294644 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Ludwin S. Department of Pathology, Kingston General Hospital, K7L 3N6, Kingston, Ontario, Canada aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 80/3(1990-07-01), 266-273 0001-6322 80:3<266 1990 80 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer