caa a22 4500 465767974 CHVBK 20180323111920.0 cr unu---uuuuu 170327e19900701xx s 000 0 eng 10.1007/BF00294642 doi (NATIONALLICENCE)springer-10.1007/BF00294642 Histological, ultrastructural and chromatographical discrimination of phospholipids in meningiomas [Elektronische Daten] [T. Yamashima, J. Yamashita] Summary: Phospholipids in meningiomas were studied by light and electron microscopy, and by high-performance liquid chromatography. They were microscopically demonstrated in six of the ten cases by Sudan III staining after the fixation with potassium dichromate. However, the conventional ultrastructural fixation with glutaraldehyde and osmium tetroxide failed to confirm phospholipids, as most of them were dissolved during dehydration. In contrast, the specimens pretreated with tannic acid before osmication ultrastructurally retained phospholipids which were represented by multilamellar bodies or ribbon-like rings. Both were found in 23 of the 30 cases within the cytoplasm, among the plasma membranes and in the extracellular matrices. The outermost lamella or ribbon showed a direct continuity from the neighbouring plasma membranes of the cytoplasm or the mitochondria. The multilamellar bodies showed an overall distribution, while the ribbon-like rings were predominantly distributed around the psammoma bodies. Precipitation of hydroxyapatite crystals within the ribbon-like rings resulted in matrix minerals of psammoma bodies. Chromatographical analyses of meningiomas disclosed phospholipids including phosphatidyl ethanolamine, phosphatidyl choline, phosphatidyl serine, sphingomyelin and phosphatidyl inositol in that order. Psammomatous meningiomas contained a higher percentage of phosphatidyl serine than non-psammomatous tumors. Ultrastructural study of synthetic phospholipids adequately treated with tannic acid showed that the multilamellar bodies were similar to phosphatidyl choline, while the ribbon-like rings were similar to phosphatidyl serine. The role of phospholipids in meningiomas is discussed. Springer-Verlag, 1990 Meningioma nationallicence Phospholipid nationallicence Psammoma nationallicence Ultrastructure nationallicence Chromatography nationallicence Yamashima T. Department of Neurosurgery, University of Kanazawa School of Medicine, Takaramachi 13-1, 920, Kanazawa City, Japan aut Yamashita J. Department of Neurosurgery, University of Kanazawa School of Medicine, Takaramachi 13-1, 920, Kanazawa City, Japan aut Acta Neuropathologica Springer-Verlag 80/3(1990-07-01), 255-259 0001-6322 80:3<255 1990 80 401 https://doi.org/10.1007/BF00294642 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00294642 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamashima T. Department of Neurosurgery, University of Kanazawa School of Medicine, Takaramachi 13-1, 920, Kanazawa City, Japan aut NATIONALLICENCE 700 1- Yamashita J. Department of Neurosurgery, University of Kanazawa School of Medicine, Takaramachi 13-1, 920, Kanazawa City, Japan aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 80/3(1990-07-01), 255-259 0001-6322 80:3<255 1990 80 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer