caa a22 4500 465768113 CHVBK 20180323111920.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00307695 doi (NATIONALLICENCE)springer-10.1007/BF00307695 Desmin and actin associated with cytoplasmic bodies in skeletal muscle fibers: immunocytochemical and fine structural studies, with a note on unusual 18- to 20-nm filaments [Elektronische Daten] [J. Schröder, C. Sommer, B. Schmidt] Summary: In a fine structural and immunocytochemical study, the latter performed on semithin sections of epoxy resin embedded skeletal muscle fibers, three types of cytoplasmic bodies were identified in a case of cytoplasmic body myopathy: (1) The first type, the classical type, showed a central core and a light halo with radiating actin filaments at the periphery. (2) The second type, the spheroid body was characterized by irregularly arranged granular masses associated with intermediate filaments. Desmin immunoreactivity occurred in the central and peripheral parts, where filaments of intermediate size were visualized by electron microscopy. Desmin immunoreactivity was noted also at the Z-bands of striated annulets, within areas of disordered myofibrils, such as sarcoplasmic masses, and in atrophic muscle fibers. (3) The third type of the cytoplasmic body was composed mainly of large masses of uneven granularity and electron density. The center of this type reacted to anti-actin antibody suggesting that the 5- to 6-nm filaments, which ultrastructurally proved to be a major component, were of the actin type. By contrast, neither intermediate filaments nor actin microfilaments were found by electron microscopy in cytoplasmic bodies in a second case where no immunoreaction to desmin or actin occurred. Anti-vimentin antibody stained only the cytoplasm of endomysial cells, but not the inclusion bodies. Some other, unusual inclusions with 18- to 20-nm tubulo-filamentous structures have to be distinguished from the various types of filaments in cytoplasmic bodies. It is concluded, that pleomorphism and heterogeneity of "cytoplasmic bodies” have to be taken into consideration when classifying cytoplasmic body myopathies. Springer-Verlag, 1990 Cytoplasmic body myopathy nationallicence Immunohistochemistry nationallicence Desmin nationallicence Intermediate filaments nationallicence Actin filaments nationallicence Schröder J. Institut für Neuropathologie der Rheinisch-Westfälischen Technischen Hochschule Aachen, Paulwelsstrasse 30, D-5100, Aachen, Federal Republic of Germany aut Sommer C. Institut für Neuropathologie der Rheinisch-Westfälischen Technischen Hochschule Aachen, Paulwelsstrasse 30, D-5100, Aachen, Federal Republic of Germany aut Schmidt B. Institut für Neuropathologie der Rheinisch-Westfälischen Technischen Hochschule Aachen, Paulwelsstrasse 30, D-5100, Aachen, Federal Republic of Germany aut Acta Neuropathologica Springer-Verlag 80/4(1990-08-01), 406-414 0001-6322 80:4<406 1990 80 401 https://doi.org/10.1007/BF00307695 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00307695 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schröder J. Institut für Neuropathologie der Rheinisch-Westfälischen Technischen Hochschule Aachen, Paulwelsstrasse 30, D-5100, Aachen, Federal Republic of Germany aut NATIONALLICENCE 700 1- Sommer C. Institut für Neuropathologie der Rheinisch-Westfälischen Technischen Hochschule Aachen, Paulwelsstrasse 30, D-5100, Aachen, Federal Republic of Germany aut NATIONALLICENCE 700 1- Schmidt B. Institut für Neuropathologie der Rheinisch-Westfälischen Technischen Hochschule Aachen, Paulwelsstrasse 30, D-5100, Aachen, Federal Republic of Germany aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 80/4(1990-08-01), 406-414 0001-6322 80:4<406 1990 80 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer