caa a22 4500 465768121 CHVBK 20180323111920.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00307690 doi (NATIONALLICENCE)springer-10.1007/BF00307690 Progressive multifocal leukoencephalopathy (PML) in AIDS and in the pre-AIDS era [Elektronische Daten] A neuropathological comparison using immunocytochemistry and in situ DNA hybridization for virus detection [M. Schmidbauer, H. Budka, K. Shah] Summary: Twenty-five brains with definite, and three brains with possible, progressive multifocal leukoencephalopathy (PML), including six brains of AIDS patients, were studied with special regard to the detection of papovaviruses. Formalin-fixed serial paraffin sections were immunostained with monospecific anti-JC virus (JCV) and genus-specific anti-simian virus (SV) 40 antisera, and hybridized in situ with DNA probes for JCV and SV 40, respectively. Immunocytochemistry (ICC) and in situ hybridization (ISH) were similarly sensitive in detecting virus in classical PML lesions. In all but one definite PML cases at least one method detected virus (96%). Possible PML tissue was never labeled. Labeling patterns were generally similar in ICC and ISH: mainly oligodendroglia and, less frequently, astroglia harbored virus, whereas labeling of neurons and endothelia was absent. Bizarre giant astrocytes were occasionally labeled by ICC and ISH. Burnt-out lesions harbored JCV DNA but not virus antigens. SV 40 DNA was never detectable. PML morphology in AIDS cases did not usually differ from the disease process seen in the pre-AIDS era. However, two AIDS brains presented extremely extended and, in one case, unusually necrotizing PML damage; in the latter case, PML lesions contained large amounts not only of JCV, but also of human immunodeficiency virus (HIV) antigens. We conclude that ICC and ISH are methods of comparable sensitivity for detection of papovavirus in flourishing PML lesions. In burnt-out PML lesions only ISH may detect virus. The possibility of an exceptional non-JCV (e.g., SV 40) etiology of PML could be neither confirmed for disproved. In AIDS, massive coinfection by HIV of PML lesions may increase damage to tissue, resulting in unusually extended and necrotizing PML. Springer-Verlag, 1990 Progressive multifocal leucoencephalopathy (PML) nationallicence AIDS nationallicence Immunocytochemistry nationallicence Nucleic acid hybridization nationallicence Papova-virus nationallicence Schmidbauer M. Neurologisches Institut, Universität Wien, Schwarzspanierstrasse 17, A-1090, Wien, Austria aut Budka H. Neurologisches Institut, Universität Wien, Schwarzspanierstrasse 17, A-1090, Wien, Austria aut Shah K. Department of Immunology and Infectious Diseases, The Johns Hopkins University School of Hygiene and Public Health, 615 N. Wolfe St., 21205, Baltimore, MD, USA aut Acta Neuropathologica Springer-Verlag 80/4(1990-08-01), 375-380 0001-6322 80:4<375 1990 80 401 https://doi.org/10.1007/BF00307690 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00307690 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schmidbauer M. Neurologisches Institut, Universität Wien, Schwarzspanierstrasse 17, A-1090, Wien, Austria aut NATIONALLICENCE 700 1- Budka H. Neurologisches Institut, Universität Wien, Schwarzspanierstrasse 17, A-1090, Wien, Austria aut NATIONALLICENCE 700 1- Shah K. Department of Immunology and Infectious Diseases, The Johns Hopkins University School of Hygiene and Public Health, 615 N. Wolfe St., 21205, Baltimore, MD, USA aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 80/4(1990-08-01), 375-380 0001-6322 80:4<375 1990 80 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer