caa a22 4500 465768199 CHVBK 20180323111921.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF00294610 doi (NATIONALLICENCE)springer-10.1007/BF00294610 Differential vulnerability of microtubule components in cerebral ischemia [Elektronische Daten] [T. Yanagihara, J. Brengman, W. Mushynski] Summary: Differential vulnerability of the major components of microtubules was examined in ischemic gerbil brains by a light microscopic, immunohisto-chemical method using monoclonal antibodies for microtubule-associated protein (MAP) 1A and MAP2, polyclonal antibody for MAP1 and 2 as well as monoclonal antibody for α-tubulin. Progressive cerebral ischemia during unilateral carotid occlusion for 5, 15 and 120 min and reperfusion for 3, 12 and 48 h following bilateral carotid occlusion for 10 min were studied. Ischemic lesions in the subiculum-CA1 region were visualized by all antibodies after ischemia for 5 min but the antibody for α-tubulin was less sensitive. The antibody for α-tubulin was also less sensitive than antibodies for MAPs for detection of early postischemic lesions. Differential sensitivity was also observed in the cerebral cortex and other brain regions. Microtubules in myelinated axons were more stable than those in dendrites. The observed loss of immunohistochemical reactivities for MAPs and α-tubulin may have been caused by activation of calcium-dependent proteolytic enzymes such as calpains. The discrepancy between MAPs and α-tubulin could be due to differences in affinities or topographic distributions of these proteins within microtubules. Springer-Verlag, 1991 Cerebral ischemia nationallicence Gerbil nationallicence Immunohis-tochemistry nationallicence Microtubule-associated proteins nationallicence Tubulin nationallicence Yanagihara T. Department of Neurology, Mayo Clinic, 55905, Rochester, MN, USA aut Brengman J. Department of Neurology, Mayo Clinic, 55905, Rochester, MN, USA aut Mushynski W. Department of Biochemistry, McGill University, Montreal, Quebec, Canada aut Acta Neuropathologica Springer-Verlag 80/5(1990-09-01), 499-505 0001-6322 80:5<499 1990 80 401 https://doi.org/10.1007/BF00294610 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00294610 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yanagihara T. Department of Neurology, Mayo Clinic, 55905, Rochester, MN, USA aut NATIONALLICENCE 700 1- Brengman J. Department of Neurology, Mayo Clinic, 55905, Rochester, MN, USA aut NATIONALLICENCE 700 1- Mushynski W. Department of Biochemistry, McGill University, Montreal, Quebec, Canada aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 80/5(1990-09-01), 499-505 0001-6322 80:5<499 1990 80 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer