caa a22 4500 465768385 CHVBK 20180323111921.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00308916 doi (NATIONALLICENCE)springer-10.1007/BF00308916 Nerve regeneration through the cryoinjured allogeneic nerve graft in the rabbit [Elektronische Daten] [K. Tohyama, C. Ide, T. Osawa] Summary: To examine whether the 3∼4-cm-long allogeneic basal lamina tubes of Schwann cells serve as conduits for regenerating axons in rabbits, allogeneic saphenous nerve, which had been predenervated and pretreated by freezing, were transplanted from Japanese White rabbits (JW) to New Zealand White rabbits (NW). Animals were killed 1, 2, 6, 8, and 14 weeks after transplantation, and the cytology at the mid-portion of the grafts was examined by electron microscopy. The distal portion of the host saphenous nerves was also examined 14 weeks after grafting. Myelin sheath debris was phagocytosed by macrophages, while the basal lamina of Schwann cells were left intact in the form of tubes. Regenerating axons were first found in such basal lamina tubes 2 weeks after grafting, and gradually increased in number. Host Schwann cells accompanied the regenerating axons behind their growing tips, separating them into individual fibers and forming thin myelin sheaths on thick axons by 6 weeks after grafting. Regenerating nerves were divided into small compartments by new perineurial cells. Newly formed blood vessels were situated outside the compartment 8 weeks after grafting. The percentage of myelinated fibers in the regenerating nerves was roughly 10% at 8 weeks and 30% at 14 weeks after grafting. The diameter of the regenerating axons, both myelinated and unmyelinated, was less than that of normal axons at all the stages examined. Numerous regenerating axons, some of which were fully myelinated, were found at the site 10 mm distal to the distal end of the graft 14 weeks after grafting. These results indicate that the Schwann cell basal lamina tubes of cryoinjured allogeneic nerves can serve as conduits for regenerating nerves in the 3∼4-cm-long graft in the rabbit. Springer-Verlag, 1990 Nerve regeneration nationallicence Allograft nationallicence Cryo-injury nationallicence Basal laminae nationallicence Rabbit nationallicence Tohyama K. Department of Anatomy School of Medicine, Iwate Medical University, 020, Morioka, Japan aut Ide C. Department of Anatomy School of Medicine, Iwate Medical University, 020, Morioka, Japan aut Osawa T. Department of Oral Anatomy School of Dentistry, Iwate Medical University, 020, Morioka, Japan aut Acta Neuropathologica Springer-Verlag 80/2(1990-06-01), 138-144 0001-6322 80:2<138 1990 80 401 https://doi.org/10.1007/BF00308916 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00308916 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tohyama K. Department of Anatomy School of Medicine, Iwate Medical University, 020, Morioka, Japan aut NATIONALLICENCE 700 1- Ide C. Department of Anatomy School of Medicine, Iwate Medical University, 020, Morioka, Japan aut NATIONALLICENCE 700 1- Osawa T. Department of Oral Anatomy School of Dentistry, Iwate Medical University, 020, Morioka, Japan aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 80/2(1990-06-01), 138-144 0001-6322 80:2<138 1990 80 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer