caa a22 4500 465768393 CHVBK 20180323111921.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00308926 doi (NATIONALLICENCE)springer-10.1007/BF00308926 Macrophage histology in paraffin-embedded multiple sclerosis plaques is demonstrated by the monoclonal pan-macrophage marker HAM-56: correlation with chronicity of the lesion [Elektronische Daten] [C. Adams, R. Poston] Summary: Using the monoclonal antibody HAM-56 with the avidin-biotin method on recent or archival paraffinembedded tissue from multiple sclerosis brains, we have been able to distinguish between acute, chronic active and inactive lesions. HAM-56 stains all macrophages, monocytes and at least some microglia; it is the only pan-macrophage marker to our knowledge that can be satisfactorily used on conventional paraffin sections. A much narrower range of mainly perivascular macrophages in acute plaques of multiple sclerosis is stained with MAC-387, anti-muramidase and anti-alpha1-antitrypsin. The acute plaques show HAM-56-stained macrophages throughout the lesion, and these macrophages exhibit profiles of phospholipid-rich myelinic bodies, which are also usually stainable with Luxol fast blue. Active ongoing lesions show a rim of macrophages at the edge of the lesion. These macrophages show profiles of large vacuoles, thought to represent the sudanophilic esterified cholesterol formed during demyelination. Inactive cases show none of these features; the few perivascular macrophages present often contain the end product of lipid peroxidation, ceroid-lipofuscin. Springer-Verlag, 1990 Demyelination nationallicence Lipids nationallicence Macrophage nationallicence Monoclonal antibody nationallicence Multiple sclerosis nationallicence Adams C. Department of Neuropathology, Runwell Hospital, SS11 7QE, Wickford, Essex, Great Britain aut Poston R. Department of Experimental Pathology, UMDS, Medical School, Guy's Hospital, SE1 9RT, London, Great Britain aut Acta Neuropathologica Springer-Verlag 80/2(1990-06-01), 208-211 0001-6322 80:2<208 1990 80 401 https://doi.org/10.1007/BF00308926 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00308926 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Adams C. Department of Neuropathology, Runwell Hospital, SS11 7QE, Wickford, Essex, Great Britain aut NATIONALLICENCE 700 1- Poston R. Department of Experimental Pathology, UMDS, Medical School, Guy's Hospital, SE1 9RT, London, Great Britain aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 80/2(1990-06-01), 208-211 0001-6322 80:2<208 1990 80 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer