caa a22 4500 465768512 CHVBK 20180323111922.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00308918 doi (NATIONALLICENCE)springer-10.1007/BF00308918 A reversible component of cerebral injury as identified by the histochemical stain 2,3,5-triphenyltetrazolium chloride (TTC) [Elektronische Daten] [D. Cole, J. Drummond, E. Ghazal, H. Shapiro] Summary: The extent of histochemical change following middle cerebral artery occlusion was quantitatively determined in three groups of Sprague-Dawley rats with 2,3,5-triphenyltetrazolium chloride (a marker of mitochondrial oxidative enzyme function). In group I (n=7) occlusion was maintained for 3 h, with immediate sacrifice. In group II (n=7) occlusion was maintained for 5 h, with immediate sacrifice. In group III (n=7) occlusion was maintained for 3 h, followed by a 2-h period of reperfusion prior to sacrifice. The area of injury was significantly larger (P<0.05) in the 5-h occlusion group [15±4% (mean±SD)] compared to the 3-h occlusion group (9±2%); indicating a time-dependent worsening of the histochemical detection of injury. However, the area of injury was significantly less in the reperfusion group (5±2%) compared to the group that was evaluated after 3 h of occlusion without reperfusion (9±2%); indicating that some component of the injury revealed by 2,3,5-triphenyltetrazolium chloride is potentially reversible. These data suggest that contrary to previous understanding, the histochemical abnormality revealed by 2,3,5-triphenyltetrazolium chloride is reversible in some circumstances and does not necessarily represent inevitable infarction. Springer-Verlag, 1990 Focal cerebral ischemia nationallicence Middle cerebral artery nationallicence Rat nationallicence Reperfusion nationallicence 2,3,5-Triphenyl-tetrazolium chloride nationallicence Cole D. Department of Anesthesiology, Loma Linda University, 92354, Loma Linda, CA, USA aut Drummond J. Department of Anesthesiology, The Veteran's Administration Medical Center, San Diego, Calif, USA aut Ghazal E. Department of Anesthesiology, Loma Linda University, 92354, Loma Linda, CA, USA aut Shapiro H. Department of Anesthesiology, The Veteran's Administration Medical Center, San Diego, Calif, USA aut Acta Neuropathologica Springer-Verlag 80/2(1990-06-01), 152-155 0001-6322 80:2<152 1990 80 401 https://doi.org/10.1007/BF00308918 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00308918 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cole D. Department of Anesthesiology, Loma Linda University, 92354, Loma Linda, CA, USA aut NATIONALLICENCE 700 1- Drummond J. Department of Anesthesiology, The Veteran's Administration Medical Center, San Diego, Calif, USA aut NATIONALLICENCE 700 1- Ghazal E. Department of Anesthesiology, Loma Linda University, 92354, Loma Linda, CA, USA aut NATIONALLICENCE 700 1- Shapiro H. Department of Anesthesiology, The Veteran's Administration Medical Center, San Diego, Calif, USA aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 80/2(1990-06-01), 152-155 0001-6322 80:2<152 1990 80 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer