caa a22 4500 465768563 CHVBK 20180323111922.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00662635 doi (NATIONALLICENCE)springer-10.1007/BF00662635 Induction of epitopes associated with neurofibrillary tangles in clonal mouse neuroblastoma (S20Y) cells [Elektronische Daten] [L. Ko, K. Sheu, O. Young, J. Blass] Summary: Accumulation of paired helical filaments (PHF) in neurofibrillary tangles is a key neuropathological hallmark in Alzheimer's disease (AD). To date, PHF have been found primarily in humans. Cultured murine cholinergic neuroblastoma (S20Y) cells, following exposure to a serum-free medium or a differentiation medium, developed immunoreactivity to anti-PHF antibodies, and to the Alz-50 by immunocytochemical and immunoblot analyses. Electron microscopic examination revealed abundant fascicles of 10-nm filaments coursing tortuously amongst organelles, such as mitochondria, endoplasmic reticulum and dense-core vesicles, in perikarya and in neuritic extensions. However, subcellular structures identical or similar to PHF could not be found in these non-human cells. This convenient cell culture model may prove to be useful for studying certain aspects of the mechanisms underlying the abnormal cytoskeletal alterations which are characteristic of AD and related neurodegenerative disorders. Springer-Verlag, 1990 Neuroblastoma cultures nationallicence Neurofibrillary tangles nationallicence Paired helical filaments nationallicence Alzheimer's disease nationallicence Ko L. Altschul Laboratory for Dementia Research, Cornell University Medical College, Burke Rehabilitation Center, 10605, White Plains, NY, USA aut Sheu K. Altschul Laboratory for Dementia Research, Cornell University Medical College, Burke Rehabilitation Center, 10605, White Plains, NY, USA aut Young O. Altschul Laboratory for Dementia Research, Cornell University Medical College, Burke Rehabilitation Center, 10605, White Plains, NY, USA aut Blass J. Altschul Laboratory for Dementia Research, Cornell University Medical College, Burke Rehabilitation Center, 10605, White Plains, NY, USA aut Acta Neuropathologica Springer-Verlag 81/1(1990-01-01), 30-40 0001-6322 81:1<30 1990 81 401 https://doi.org/10.1007/BF00662635 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00662635 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ko L. Altschul Laboratory for Dementia Research, Cornell University Medical College, Burke Rehabilitation Center, 10605, White Plains, NY, USA aut NATIONALLICENCE 700 1- Sheu K. Altschul Laboratory for Dementia Research, Cornell University Medical College, Burke Rehabilitation Center, 10605, White Plains, NY, USA aut NATIONALLICENCE 700 1- Young O. Altschul Laboratory for Dementia Research, Cornell University Medical College, Burke Rehabilitation Center, 10605, White Plains, NY, USA aut NATIONALLICENCE 700 1- Blass J. Altschul Laboratory for Dementia Research, Cornell University Medical College, Burke Rehabilitation Center, 10605, White Plains, NY, USA aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 81/1(1990-01-01), 30-40 0001-6322 81:1<30 1990 81 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer