caa a22 4500 465768717 CHVBK 20180323111922.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00662630 doi (NATIONALLICENCE)springer-10.1007/BF00662630 In situ hybridization of muscle mitochondrial mRNA in mitochondrial myopathies [Elektronische Daten] [S. Nakamura, T. Sato, H. Hirawake, R. Kobayashi, Y. Fukuda, J. Kawamura, H. Ujike, S. Horai] Summary: To determine whether a mitochondrial mRNA deficiency exists in mitochondrial myopathies, muscle biopsies from a patient with chronic progressive external ophthalmoplegia (CPEO) and a patient with mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS) were studied using in situ hybridization. Histochemistry and immunohistochemistry were performed along with hybridization. Hybridization reactions were widely distributed over the sarcoplasm of all muscle fibers in the patient with MELAS. In the patient with CPEO, 80% of the fibers showed a marked decrease in density of autoradiographic grains. This marked decrease corresponded to the histochemical and immunohistochemical findings of a very weak staining of cytochromec oxidase (CCO). The isotope-labeled cDNA probe used in in situ hybridization in this study complements a part of subunit I of CCO and a part of subunit II of complex I in the mitochondrial gene. Our results suggest a defect in the mRNA in this CPEO patient. Springer-Verlag, 1990 In situ hybridization nationallicence Mitochondrial DNA nationallicence Mitochondrial myopathy nationallicence Ophthalmoplegia nationallicence Cytochrome c oxidase nationallicence Nakamura S. Department of Pathology, Juntendo University School of Medicine, 2-1 Hongo, 113, Tokyo, Japan aut Sato T. Department of Neurology, Juntendo University School of Medicine, 2-1 Hongo, 113, Tokyo, Japan aut Hirawake H. Department of Neurology, Juntendo University School of Medicine, 2-1 Hongo, 113, Tokyo, Japan aut Kobayashi R. Department of Forensic Medicine, Juntendo University School of Medicine, 2-1 Hongo, 113, Tokyo, Japan aut Fukuda Y. Department of Pathology, Juntendo University School of Medicine, 2-1 Hongo, 113, Tokyo, Japan aut Kawamura J. Department of Neurology, Tenri Hospital, Tenri, Japan aut Ujike H. Department of Psychiatry, Okayama University, Okayama, Japan aut Horai S. National Institute of Genetics, Mishima, Japan aut Acta Neuropathologica Springer-Verlag 81/1(1990-01-01), 1-6 0001-6322 81:1<1 1990 81 401 https://doi.org/10.1007/BF00662630 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00662630 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakamura S. Department of Pathology, Juntendo University School of Medicine, 2-1 Hongo, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Sato T. Department of Neurology, Juntendo University School of Medicine, 2-1 Hongo, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Hirawake H. Department of Neurology, Juntendo University School of Medicine, 2-1 Hongo, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Kobayashi R. Department of Forensic Medicine, Juntendo University School of Medicine, 2-1 Hongo, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Fukuda Y. Department of Pathology, Juntendo University School of Medicine, 2-1 Hongo, 113, Tokyo, Japan aut NATIONALLICENCE 700 1- Kawamura J. Department of Neurology, Tenri Hospital, Tenri, Japan aut NATIONALLICENCE 700 1- Ujike H. Department of Psychiatry, Okayama University, Okayama, Japan aut NATIONALLICENCE 700 1- Horai S. National Institute of Genetics, Mishima, Japan aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 81/1(1990-01-01), 1-6 0001-6322 81:1<1 1990 81 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer