caa a22 4500 465768792 CHVBK 20180323111923.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00334501 doi (NATIONALLICENCE)springer-10.1007/BF00334501 Interaction between human brain tumour biopsies and fetal rat brain tissue in vitro [Elektronische Daten] [O. Engebraaten, R. Bjerkvig, M. Lund-Johansen, K. Wester, P.-H. Pedersen, S. Mork, E.-O. Backlund, O. Laerum] Summary: The invasiveness of human intracranial tumours was studied in an organ culture system. Biopsies from six glioblastomas, four astrocytomas, two mixed gliomas, one ependymoma, four meningiomas and two carcinoma metastases were cut into fragments of 0.5 mm diameter, and placed in agar overlay tissue culture. The tumour specimens formed spheroids which were co-cultured with cell aggregates or fragments from fetal rat brain for up to 10 days in vitro. The invasiveness of the glioblastoma spheroids was characterised by a gradual destruction of normal brain tissue by tumour cells, followed by replacement of normal tissue by these cells. Cocultures from two glioblastomas showed lesions in the normal brain tissue in areas removed from the tumour cells. Tumour spheroids from four glioblastomas totally destroyed the normal brain tissue without any change in the original tumour spheroid configuration. The lowgrade gliomas were less invasive than the glioblastomas. The meningiomas and the metastases were non-invasive. This organ culture assay appeared to reflect the in situ invasive behaviour of the brain tumours examined. It is suggested that it may be used for evaluating the aggressiveness of individual brain tumours with the specific aim of correlating clinical data with the biological character of the tumour. Springer-Verlag, 1990 Biopsy nationallicence Brain nationallicence Glioma nationallicence Neoplasm invasiveness nationallicence Organ culture nationallicence Engebraaten O. The Gade Institute, Department of Pathology, University of Bergen, School of Medicine, Haukeland Hospital, N-5021, Norway aut Bjerkvig R. The Gade Institute, Department of Pathology, University of Bergen, School of Medicine, Haukeland Hospital, N-5021, Norway aut Lund-Johansen M. The Gade Institute, Department of Pathology, University of Bergen, School of Medicine, Haukeland Hospital, N-5021, Norway aut Wester K. Department of Neurosurgery, University of Bergen, School of Medicine, Haukeland Hospital, N-5021, Norway aut Pedersen P.-H Department of Neurosurgery, University of Bergen, School of Medicine, Haukeland Hospital, N-5021, Norway aut Mork S. The Gade Institute, Department of Pathology, University of Bergen, School of Medicine, Haukeland Hospital, N-5021, Norway aut Backlund E.-O Department of Neurosurgery, University of Bergen, School of Medicine, Haukeland Hospital, N-5021, Norway aut Laerum O. The Gade Institute, Department of Pathology, University of Bergen, School of Medicine, Haukeland Hospital, N-5021, Norway aut Acta Neuropathologica Springer-Verlag 81/2(1990-12-01), 130-140 0001-6322 81:2<130 1990 81 401 https://doi.org/10.1007/BF00334501 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00334501 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Engebraaten O. The Gade Institute, Department of Pathology, University of Bergen, School of Medicine, Haukeland Hospital, N-5021, Norway aut NATIONALLICENCE 700 1- Bjerkvig R. The Gade Institute, Department of Pathology, University of Bergen, School of Medicine, Haukeland Hospital, N-5021, Norway aut NATIONALLICENCE 700 1- Lund-Johansen M. The Gade Institute, Department of Pathology, University of Bergen, School of Medicine, Haukeland Hospital, N-5021, Norway aut NATIONALLICENCE 700 1- Wester K. Department of Neurosurgery, University of Bergen, School of Medicine, Haukeland Hospital, N-5021, Norway aut NATIONALLICENCE 700 1- Pedersen P.-H Department of Neurosurgery, University of Bergen, School of Medicine, Haukeland Hospital, N-5021, Norway aut NATIONALLICENCE 700 1- Mork S. The Gade Institute, Department of Pathology, University of Bergen, School of Medicine, Haukeland Hospital, N-5021, Norway aut NATIONALLICENCE 700 1- Backlund E.-O Department of Neurosurgery, University of Bergen, School of Medicine, Haukeland Hospital, N-5021, Norway aut NATIONALLICENCE 700 1- Laerum O. The Gade Institute, Department of Pathology, University of Bergen, School of Medicine, Haukeland Hospital, N-5021, Norway aut NATIONALLICENCE 773 0- Acta Neuropathologica Springer-Verlag 81/2(1990-12-01), 130-140 0001-6322 81:2<130 1990 81 401 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer