caa a22 4500 465769446 CHVBK 20180323111925.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00151713 doi (NATIONALLICENCE)springer-10.1007/BF00151713 Establishment of stable mouse/human-human hybrid cell lines producing large amounts of anti-tetanus human monoclonal antibodies with high neutralizing activity [Elektronische Daten] [M. Kamei, S. Hashizume, N. Sugimoto, K. Ozutsumi, M. Matsuda] To establish stable hybrid cell lines producing human anti-tetanus antibody with high toxinneutralizing activity, peripheral lymphocytes from humans hyperimmunized with tetanus toxoid were, after in vitro antigen stimulation, fused with a mouse/human heteromyeloma or human lymphoblastoid cell line and cloned. Unlike the IgM secretors (six clones), the IgG secretors we obtained (six clones) produced anti-tetanus human monoclonal antibodies with high neutralizing activity (the highest one, cell line G2, 4.3 IU/100 μg IgG). Appropriate combinations of three or four kinds of monoclonal antibodies of the IgG type resulted in markedly increased neutralizing activity comparable with that of anti-tetanus human polyclonal immunoglobulin preparations currently used clinically on the basis of toxin-specific IgG content. Five of these cell lines produced 10≈20 pg of antibody per ml for more than 3 months. The cell line G2 produced 6 mg of antibody per day in serum-free medium in a 500-ml bioreactor in perfusion culture and 13-104 mg in a nude mouse. These cell lines satisfied, for the first time, the minimal requirements for applying human monoclonal antibodies to clinical use. Kluwer Academic Publishers, 1990 Hybrid cell lines nationallicence Anti-tetanus monoclonal antibodies nationallicence Human monoclonal antibodies nationallicence Neutralizing antibodies nationallicence Kamei M. Morinaga Institute of Biological Science, 2-1-1 Shimosueyoshi, Tsurumi-ku, 230, Yokohama, Kanagawa, Japan aut Hashizume S. Morinaga Institute of Biological Science, 2-1-1 Shimosueyoshi, Tsurumi-ku, 230, Yokohama, Kanagawa, Japan aut Sugimoto N. Department of Tuberculosis Research, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, 565, Osaka, Japan aut Ozutsumi K. Department of Tuberculosis Research, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, 565, Osaka, Japan aut Matsuda M. Department of Tuberculosis Research, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, 565, Osaka, Japan aut European Journal of Epidemiology Kluwer Academic Publishers 6/4(1990-12-01), 386-397 0393-2990 6:4<386 1990 6 10654 https://doi.org/10.1007/BF00151713 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00151713 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kamei M. Morinaga Institute of Biological Science, 2-1-1 Shimosueyoshi, Tsurumi-ku, 230, Yokohama, Kanagawa, Japan aut NATIONALLICENCE 700 1- Hashizume S. Morinaga Institute of Biological Science, 2-1-1 Shimosueyoshi, Tsurumi-ku, 230, Yokohama, Kanagawa, Japan aut NATIONALLICENCE 700 1- Sugimoto N. Department of Tuberculosis Research, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, 565, Osaka, Japan aut NATIONALLICENCE 700 1- Ozutsumi K. Department of Tuberculosis Research, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, 565, Osaka, Japan aut NATIONALLICENCE 700 1- Matsuda M. Department of Tuberculosis Research, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, 565, Osaka, Japan aut NATIONALLICENCE 773 0- European Journal of Epidemiology Kluwer Academic Publishers 6/4(1990-12-01), 386-397 0393-2990 6:4<386 1990 6 10654 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer