caa a22 4500 465771572 CHVBK 20180323111930.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00179631 doi (NATIONALLICENCE)springer-10.1007/BF00179631 Both gonadotropin and testosterone fail to reverse estrogen-induced cryptorchidism in fetal mice: further evidence for nonandrogenic control of testicular descent in the fetus [Elektronische Daten] [John Hutson, Lisa Watts, J. Montalto, S. Greco] Estradiol benzoate (E2B) causes cryptorchidism in fetal mice, with suppression of androgen secretion. Simultaneous gonadotropin (hCG) injection was reported to restore Leydig cell function and testosterone mediates testicular descent. To investigate whether hCG or testosterone fully reverses the cryptorchidism as well as testosterone secretion, fetal mice were exposed to E2B with or without hCG/testosterone. Normal transabdominal testicular descent was significantly inhibited by E2B or diethylstilbestrol injection on day 14 of gestation (n = 41; P < 0.005). Both hCG 50-100 IU (n = 59) and testosterone 5 mg (n = 45) given simultaneously with E2B failed to restore normal testicular descent (P < 0.005), with the testicular position the same as after E2B treatment alone. Mean testicular testosterone content at birth after E2B administration was 66±55 pg/testis (n = 15), while following E2B plus hCG it was 115±58 pg/testis (n = 10). Normal control mice had a testicular testosterone content of 882±422 pg/testis (n = 12). These results make the view that hCG or testosterone can reverse estrogen-induced cryptorchidism in the fetus questionable. It is proposed that estrogen not only suppresses the hypothalamic-pituitary axis and androgen secretion, but also has a direct inhibitory effect on testicular descent unrelated to androgen action. The action of müllerian inhibiting substance is known to be inhibited by estrogen during müllerian duct regression, and this may be the mechanism by which estrogen inhibits testicular descent. Springer-Verlag, 1990 Cryptorchidism nationallicence Fetal mouse nationallicence Estradiol benzoate nationallicence Estrogen nationallicence hCG nationallicence Hutson John Department of Pediatrics, University of Melbourne, Melbourne, Victoria, Australia aut Watts Lisa Research Foundation and Departments of General Surgery and Clinical Biochemistry, Royal Children's Hospital, 3052, Melbourne Parkville, Victoria, Australia aut Montalto J. Research Foundation and Departments of General Surgery and Clinical Biochemistry, Royal Children's Hospital, 3052, Melbourne Parkville, Victoria, Australia aut Greco S. Research Foundation and Departments of General Surgery and Clinical Biochemistry, Royal Children's Hospital, 3052, Melbourne Parkville, Victoria, Australia aut Pediatric Surgery International Springer-Verlag 5/1(1990-01-01), 13-18 0179-0358 5:1<13 1990 5 383 https://doi.org/10.1007/BF00179631 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00179631 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hutson John Department of Pediatrics, University of Melbourne, Melbourne, Victoria, Australia aut NATIONALLICENCE 700 1- Watts Lisa Research Foundation and Departments of General Surgery and Clinical Biochemistry, Royal Children's Hospital, 3052, Melbourne Parkville, Victoria, Australia aut NATIONALLICENCE 700 1- Montalto J. Research Foundation and Departments of General Surgery and Clinical Biochemistry, Royal Children's Hospital, 3052, Melbourne Parkville, Victoria, Australia aut NATIONALLICENCE 700 1- Greco S. Research Foundation and Departments of General Surgery and Clinical Biochemistry, Royal Children's Hospital, 3052, Melbourne Parkville, Victoria, Australia aut NATIONALLICENCE 773 0- Pediatric Surgery International Springer-Verlag 5/1(1990-01-01), 13-18 0179-0358 5:1<13 1990 5 383 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer