caa a22 4500 465772986 CHVBK 20180323111934.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00505641 doi (NATIONALLICENCE)springer-10.1007/BF00505641 A new model of epidermal differentiation: Induction by mechanical stimulation [Elektronische Daten] [F. Görmar, A. Bernd, J. Bereiter-Hahn, H. Holzmann] Summary: In vivo, epidermal cells are committed to terminal differentiation in which they undergo a series of morphological and biochemical changes. In vitro, keratinocytes are able to undergo some steps of this differentiation process only. In view of the fact that in vivo skin is continuously subjected to mechanical stress, we investigated the stimulation of differentiation of transformed keratinocytes by mechanical stimulation. The cells, grown in plastic culture dishes, were periodically treated with weights exerting a pressure of 0.015 Ncm−2. This stimulation lasted from 1 to 4 days. Then keratinocytes were examined using indirect immunofluorescence, 3H-thymidine and 14C-amino acid incorporation, SDS polyacrylamide gel electrophoresis, and Western blotting. Following pressure treatment, the previously monolayered keratinocytes locally grew up to several layers, the number of horny scales increased and, after 4 days, the pattern of cytokeratin was modified. The total amount of keratin increased, forming granular accumulations, while the proliferation rate of the cells decreased. Both the 67 kDa and 49.5 kDa keratin subunits increased in stimulated cells. Moreover, a weak keratin band of 44 kDa appeared that was not present in controls. The results demonstrate that cyclic pressure promotes differentiation of cultivated epidermal cells. Springer-Verlag, 1990 Epidermal differentiation nationallicence Keratinocytes nationallicence Mechanical stimulation nationallicence Görmar F. Zentrum der Dermatologie und Venerologie, J. W. Goethe-UniversitÄt, Theodor-Stern-Kai 7, D-6000, Frankfurt (M) 70, Federal Republic of Germany aut Bernd A. Zentrum der Dermatologie und Venerologie, J. W. Goethe-UniversitÄt, Theodor-Stern-Kai 7, D-6000, Frankfurt (M) 70, Federal Republic of Germany aut Bereiter-Hahn J. AK Kinematische Zellforschung, J. W. Goethe-UniversitÄt, Senckenberganlage 27, D-6000, Frankfurt (M) 11, Federal Republic of Germany aut Holzmann H. Zentrum der Dermatologie und Venerologie, J. W. Goethe-UniversitÄt, Theodor-Stern-Kai 7, D-6000, Frankfurt (M) 70, Federal Republic of Germany aut Archives of Dermatological Research Springer-Verlag 282/1(1990-01-01), 22-32 0340-3696 282:1<22 1990 282 403 https://doi.org/10.1007/BF00505641 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00505641 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Görmar F. Zentrum der Dermatologie und Venerologie, J. W. Goethe-UniversitÄt, Theodor-Stern-Kai 7, D-6000, Frankfurt (M) 70, Federal Republic of Germany aut NATIONALLICENCE 700 1- Bernd A. Zentrum der Dermatologie und Venerologie, J. W. Goethe-UniversitÄt, Theodor-Stern-Kai 7, D-6000, Frankfurt (M) 70, Federal Republic of Germany aut NATIONALLICENCE 700 1- Bereiter-Hahn J. AK Kinematische Zellforschung, J. W. Goethe-UniversitÄt, Senckenberganlage 27, D-6000, Frankfurt (M) 11, Federal Republic of Germany aut NATIONALLICENCE 700 1- Holzmann H. Zentrum der Dermatologie und Venerologie, J. W. Goethe-UniversitÄt, Theodor-Stern-Kai 7, D-6000, Frankfurt (M) 70, Federal Republic of Germany aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/1(1990-01-01), 22-32 0340-3696 282:1<22 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer