caa a22 4500 465773001 CHVBK 20180323111934.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00505639 doi (NATIONALLICENCE)springer-10.1007/BF00505639 Ultrastructural localization of keratin and α- l -fucose in human eccrine sweat glands [Elektronische Daten] [G. Metzler, G. Schaumburg-Lever, K. Liebig] Summary: Human eccrine sweat glands were embedded in Lowicryl K4M. Cytokeratin proteins and blood group H antigen were localized by applying a postembedding immunogold method using a monoclonal antikeratin antibody and the lectin Ulex europaeus I. The antikeratin antibody labeled intermediate filaments in the secretory coil and dermal duct. Within dark secretory cells bundles of filaments criss-crossing the cell were labeled. Within the luminal cells of the dermal duct filaments arranged parallel to the cell surface and lying in the apex of the cell were labeled, too. The association of keratin filaments with desmosomes was visualized demonstrating their subcellular connection with other cell organelles. The desmosomes themselves remained unlabeled. The lectin Ulex europaeus I is a blood group H specific lectin and binds to α-l-fucosyl-containing glycoproteins. Dark cells of the secretory coil reacted with the lectin. Here the secretory granules, the lateral cell membranes, and the microvilli membranes were labeled. The endoplasmatic reticulum, the Golgi complex, and transport vesicles were not labeled, although the glycoprotein synthesis is considered to be located in the Golgi complex. Thus, either the number of α-l-fucose molecules in the Golgi is too low to be detected by the technique employed or the determinant of blood group H antigen is released after the secretory granules and transport vesicles leave the Golgi complex. Springer-Verlag, 1990 Immunogold nationallicence Electron microscopy nationallicence Eccrine sweat glands nationallicence Keratin nationallicence Fucose nationallicence Metzler G. Department of Dermatology, Eberhard-Karls-UniversitÄt, Liebermeisterstr. 25, D-7400, Tübingen, Federal Republic of Germany aut Schaumburg-Lever G. Department of Dermatology, Eberhard-Karls-UniversitÄt, Liebermeisterstr. 25, D-7400, Tübingen, Federal Republic of Germany aut Liebig K. Department of Dermatology, Eberhard-Karls-UniversitÄt, Liebermeisterstr. 25, D-7400, Tübingen, Federal Republic of Germany aut Archives of Dermatological Research Springer-Verlag 282/1(1990-01-01), 12-16 0340-3696 282:1<12 1990 282 403 https://doi.org/10.1007/BF00505639 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00505639 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Metzler G. Department of Dermatology, Eberhard-Karls-UniversitÄt, Liebermeisterstr. 25, D-7400, Tübingen, Federal Republic of Germany aut NATIONALLICENCE 700 1- Schaumburg-Lever G. Department of Dermatology, Eberhard-Karls-UniversitÄt, Liebermeisterstr. 25, D-7400, Tübingen, Federal Republic of Germany aut NATIONALLICENCE 700 1- Liebig K. Department of Dermatology, Eberhard-Karls-UniversitÄt, Liebermeisterstr. 25, D-7400, Tübingen, Federal Republic of Germany aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/1(1990-01-01), 12-16 0340-3696 282:1<12 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer