caa a22 4500 465773060 CHVBK 20180323111934.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF00372092 doi (NATIONALLICENCE)springer-10.1007/BF00372092 Electron microscopic study of cultured cells from the murine hair tissues: cell growth and differentiation [Elektronische Daten] [N. Tanigaki, H. Ando, M. Ito, A. Hashimoto, Y. Kitano] Summary: The cultured hair cells from 4-day-old C3H mice were studied by electron microscopy. The hair roots isolated from the skin by collagenase digestion were dispersed into a cell suspension by treatment with a mixture of trypsin and ethylenediaminetetraacetate. The cells were cultured in MCDB-153 (a medium containing seven growth factors) for 1, 3, 6 or 13 days. The number of cultured cells on day 3 was twice that on day 1, and stayed at the same level until day 13. By electron microscopy, some of the cells cultured for 1 day were seen to be undifferentiated and others already showed differentiation into various hair structures. Such differentiated cells disappeared on day 3 and most of the cells cultured for 3 days were undifferentiated. Cells cultured for 6 days were differentiated showing inner root sheath cell, hair cortical cell and medulla cell structures. The characteristics of these cultured cells corresponded well to those of in vivo cells of the hair tissues from the back skin of 7-day-old C3H mice. On day 13 degeneration occurred in the cultured cells. In none of these cultures were mesenchymal cells, such as fibroblasts, found. The present electron microscopic study reveals that immature cells obtained from mouse hair tissues proliferate in vitro and differentiate into several subpopulations corresponding to those of in vivo cell layers of hair tissues. The present culture technique may be useful for studies of hair cell growth and differentiation. Springer-Verlag, 1990 Electron microscopy nationallicence Culture nationallicence Hair cells nationallicence Growth nationallicence Differentiation nationallicence Tanigaki N. Fundamental Research Laboratory, R&D Division Sunstar Inc., 3-1, Asahi-Machi, Takatsuki, 569, Osaka, Japan aut Ando H. Fundamental Research Laboratory, R&D Division Sunstar Inc., 3-1, Asahi-Machi, Takatsuki, 569, Osaka, Japan aut Ito M. Department of Dermatology, Niigata University School of Medicine, Niigata, Japan aut Hashimoto A. Fundamental Research Laboratory, R&D Division Sunstar Inc., 3-1, Asahi-Machi, Takatsuki, 569, Osaka, Japan aut Kitano Y. Department of Dermatology, Hyogo College of Medicine, Nishinomiya, Japan aut Archives of Dermatological Research Springer-Verlag 282/6(1990-09-01), 402-407 0340-3696 282:6<402 1990 282 403 https://doi.org/10.1007/BF00372092 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00372092 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tanigaki N. Fundamental Research Laboratory, R&D Division Sunstar Inc., 3-1, Asahi-Machi, Takatsuki, 569, Osaka, Japan aut NATIONALLICENCE 700 1- Ando H. Fundamental Research Laboratory, R&D Division Sunstar Inc., 3-1, Asahi-Machi, Takatsuki, 569, Osaka, Japan aut NATIONALLICENCE 700 1- Ito M. Department of Dermatology, Niigata University School of Medicine, Niigata, Japan aut NATIONALLICENCE 700 1- Hashimoto A. Fundamental Research Laboratory, R&D Division Sunstar Inc., 3-1, Asahi-Machi, Takatsuki, 569, Osaka, Japan aut NATIONALLICENCE 700 1- Kitano Y. Department of Dermatology, Hyogo College of Medicine, Nishinomiya, Japan aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/6(1990-09-01), 402-407 0340-3696 282:6<402 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer