caa a22 4500 465773141 CHVBK 20180323111934.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF00372088 doi (NATIONALLICENCE)springer-10.1007/BF00372088 Interleukin-1 induces collagenase production by recessive dystrophic epidermolysis bullosa fibroblasts [Elektronische Daten] [K. Nomura, T. Imaizumi, H. Mikami, K. Tamai, M. Takahashi, I. Hashimoto] Summary: We examined the direct effect of interleukin-1 (IL-1) on the collagenase production by epidermolysis bullosa (EB) fibroblasts. Addition of IL-1 at concentrations of 2.5×10−4 units/ml or below in the culture media greatly enhanced collagenase production by two cell lines of recessive dystrophic EB (RDEB) fibroblasts. They produced 4.82±0.04 to 5.93±0.39 units/ml of enzyme, as compared to 0.02±0.07 units/ml in the absence of IL-1. In contrast, collagenase production by two cell lines of dominant dystrophic EB (DDEB) and normal fibroblasts was not, or only slightly, increased up to 0.69±0.28 units/ml. IL-1 concentrations of 2.5×10−3 units/ml or higher failed to induce collagenase production by all fibroblasts. 3H-thymidine uptake increased by about 110-376% of control after IL-1 treatment. In addition, these data were obtained using fibroblasts of the 13-15 passages, suggesting that the property might be determined genetically. Although RDEB seems to be a wide heterogeneous group, the present data strongly suggest that the property may be specific to and characteristic of some types of RDEB cells. Springer-Verlag, 1990 Epidermolysis bullosa nationallicence Collagenase nationallicence Interleukin-1 nationallicence Nomura K. Department of Dermatology, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut Imaizumi T. Department of Dermatology, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut Mikami H. Department of Dermatology, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut Tamai K. Department of Dermatology, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut Takahashi M. Department of Dermatology, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut Hashimoto I. Department of Dermatology, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut Archives of Dermatological Research Springer-Verlag 282/6(1990-09-01), 379-382 0340-3696 282:6<379 1990 282 403 https://doi.org/10.1007/BF00372088 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00372088 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nomura K. Department of Dermatology, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut NATIONALLICENCE 700 1- Imaizumi T. Department of Dermatology, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut NATIONALLICENCE 700 1- Mikami H. Department of Dermatology, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut NATIONALLICENCE 700 1- Tamai K. Department of Dermatology, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut NATIONALLICENCE 700 1- Takahashi M. Department of Dermatology, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut NATIONALLICENCE 700 1- Hashimoto I. Department of Dermatology, Hirosaki University School of Medicine, 5 Zaifu-cho, 036, Hirosaki, Japan aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/6(1990-09-01), 379-382 0340-3696 282:6<379 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer