caa a22 4500 465773206 CHVBK 20180323111934.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00402620 doi (NATIONALLICENCE)springer-10.1007/BF00402620 Human papillomavirus DNA from warts for typing by endonuclease restriction patterns: purification by alkaline plasmid methods [Elektronische Daten] [M. Chinami, E. Tanikawa, H. Hachisuka, Y. Sasai, M. Shingu] Summary: The alkaline plasmid DNA extraction method of Birnboim and Doly was applied for the isolation of human papillomavirus (HPV) from warts. Tissue from common and plantar warts was digested with proteinase K, and the extrachromosomal circular covalently-closed form of HPV-DNA was rapidly extracted by alkaline sodium dodecyl sulphate and phenol-chloroform treatment. Recovery of HPV-DNA from the tissue was sufficient for determination of endonuclease restriction patterns by agarose gel electrophoresis. Springer-Verlag, 1990 Human papillomavirus nationallicence Verruca nationallicence DNA typing nationallicence Chinami M. Department of Virology, Kurume University School of Medicine, 67 Asahimachi Kurume, 830, Japan aut Tanikawa E. Department of Virology, Kurume University School of Medicine, 67 Asahimachi Kurume, 830, Japan aut Hachisuka H. Cutaneous Biology Unit, Department of Dermatology, Kurume University School of Medicine, 67 Asahimachi Kurume, 830, Japan aut Sasai Y. Cutaneous Biology Unit, Department of Dermatology, Kurume University School of Medicine, 67 Asahimachi Kurume, 830, Japan aut Shingu M. Department of Virology, Kurume University School of Medicine, 67 Asahimachi Kurume, 830, Japan aut Archives of Dermatological Research Springer-Verlag 282/7(1990-10-01), 446-448 0340-3696 282:7<446 1990 282 403 https://doi.org/10.1007/BF00402620 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00402620 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chinami M. Department of Virology, Kurume University School of Medicine, 67 Asahimachi Kurume, 830, Japan aut NATIONALLICENCE 700 1- Tanikawa E. Department of Virology, Kurume University School of Medicine, 67 Asahimachi Kurume, 830, Japan aut NATIONALLICENCE 700 1- Hachisuka H. Cutaneous Biology Unit, Department of Dermatology, Kurume University School of Medicine, 67 Asahimachi Kurume, 830, Japan aut NATIONALLICENCE 700 1- Sasai Y. Cutaneous Biology Unit, Department of Dermatology, Kurume University School of Medicine, 67 Asahimachi Kurume, 830, Japan aut NATIONALLICENCE 700 1- Shingu M. Department of Virology, Kurume University School of Medicine, 67 Asahimachi Kurume, 830, Japan aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/7(1990-10-01), 446-448 0340-3696 282:7<446 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer