caa a22 4500 465773257 CHVBK 20180323111934.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00402622 doi (NATIONALLICENCE)springer-10.1007/BF00402622 Sensitizing capacity of three methyl alkanesulphonates: a murine in vivo and in vitro model of allergic contact dermatitis [Elektronische Daten] [R. Fraginals, J. Lepoittevin, C. Benezra] Summary: A murine model for in vivo and in vitro studies of contact sensitization to methyl alkanesulphonate derivatives has been developed. Contact sensitivity was quantified in vivo by measuring the ear thickness increase, and the influence of the alkyl chain length (hexyl, dodecyl, hexadecyl) was investigated. Long chain methyl alkanesulphonates (dodecyl and hexadecyl) are strong sensitizers, while the shorter alkyl chain, methyl hexanesulphonate, is a weak sensitizer. In vitro lymphocyte blastogenesis was measured by the 3H-thymidine uptake and exhibited a stimulation index between 2 and 8. The results nicely parallelled the in vivo sensitization measurements, except for methyl dodecanesulphonate. This could be explained by the cytotoxic activity of this compound, the most toxic of the three methyl alkanesulphonates tested. Thus, murine sensitization to methyl alkanesulphonates provides a good model system for preliminary investigations of delayed type hypersensitivity mechanisms. Springer-Verlag, 1990 Methyl alkanesulphonates nationallicence Murine model system nationallicence Delayed hypersensitivity nationallicence Allergic contact dermatitis nationallicence Fraginals R. Laboratoire de Dermatochimie associé au CNRS, Université Louis Pasteur, Clinique Dermatologique, CHU, F-67091, Strasbourg, France aut Lepoittevin J. Laboratoire de Dermatochimie associé au CNRS, Université Louis Pasteur, Clinique Dermatologique, CHU, F-67091, Strasbourg, France aut Benezra C. Laboratoire de Dermatochimie associé au CNRS, Université Louis Pasteur, Clinique Dermatologique, CHU, F-67091, Strasbourg, France aut Archives of Dermatological Research Springer-Verlag 282/7(1990-10-01), 455-458 0340-3696 282:7<455 1990 282 403 https://doi.org/10.1007/BF00402622 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00402622 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fraginals R. Laboratoire de Dermatochimie associé au CNRS, Université Louis Pasteur, Clinique Dermatologique, CHU, F-67091, Strasbourg, France aut NATIONALLICENCE 700 1- Lepoittevin J. Laboratoire de Dermatochimie associé au CNRS, Université Louis Pasteur, Clinique Dermatologique, CHU, F-67091, Strasbourg, France aut NATIONALLICENCE 700 1- Benezra C. Laboratoire de Dermatochimie associé au CNRS, Université Louis Pasteur, Clinique Dermatologique, CHU, F-67091, Strasbourg, France aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/7(1990-10-01), 455-458 0340-3696 282:7<455 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer