caa a22 4500 465773265 CHVBK 20180323111934.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00402621 doi (NATIONALLICENCE)springer-10.1007/BF00402621 The inhibition of free radical generation by human neutrophils through the synergistic effects of metronidazole with palmitoleic acid: a possible mechanism of action of metronidazole in rosacea and acne [Elektronische Daten] [H. Akamatsu, M. Oguchi, S. Nishijima, Y. Asada, M. Takahashi, T. Ushijima, Y. Niwa] Summary: Metronidazole is clinically effective in treating not only rosacea but also acne inflammation. Yet it is generally considered not to be very effective in inhibiting the growth of anaerobic Propionibacterium acnes. We report here our investigation into the synergistic effects of metronidazole and palmitoleic acid on the anaerobic growth of P. acnes as well as on human neutrophil functions, including the generation of reactive oxygen species (ROS). Both metronidazole and palmitoleic acid, when used alone, only slightly inhibited the growth of P. acnes, and no significant decrease in human neutrophil functions, including the generation of ROS, was observed. But metronidazole used in the presence of palmitoleic acid markedly inhibited the anaerobic growth of P. acnes and decreased ROS generation by neutrophils. However, ROS generated in the xanthine-xanthine oxidase system were not affected. Metronidazole was shown to be clinically effective by decreasing neutrophil-generated ROS at the sites of inflammation with the aid of palmitoleic acid, which is generally present in human skin. By inhibiting oxidative tissue injury under in vivo conditions, treatment with metronidazole results in remarkable improvement of rosacea and acne. Springer-Verlag, 1990 Metronidazole nationallicence Palmitoleic acid nationallicence Free radical inhibitory effect nationallicence Rosacea nationallicence Acne nationallicence Akamatsu H. Department of Dermatology, Kansai Medical University, 570, Moriguchi aut Oguchi M. Department of Dermatology, Kansai Medical University, 570, Moriguchi aut Nishijima S. Department of Dermatology, Kansai Medical University, 570, Moriguchi aut Asada Y. Department of Dermatology, Kansai Medical University, 570, Moriguchi aut Takahashi M. Department of Microbiology, Shiga University of Medical Science, 520-21, Otsu, Japan aut Ushijima T. Department of Microbiology, Shiga University of Medical Science, 520-21, Otsu, Japan aut Niwa Y. Department of Dermatology, Kansai Medical University, 570, Moriguchi aut Archives of Dermatological Research Springer-Verlag 282/7(1990-10-01), 449-454 0340-3696 282:7<449 1990 282 403 https://doi.org/10.1007/BF00402621 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00402621 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Akamatsu H. Department of Dermatology, Kansai Medical University, 570, Moriguchi aut NATIONALLICENCE 700 1- Oguchi M. Department of Dermatology, Kansai Medical University, 570, Moriguchi aut NATIONALLICENCE 700 1- Nishijima S. Department of Dermatology, Kansai Medical University, 570, Moriguchi aut NATIONALLICENCE 700 1- Asada Y. Department of Dermatology, Kansai Medical University, 570, Moriguchi aut NATIONALLICENCE 700 1- Takahashi M. Department of Microbiology, Shiga University of Medical Science, 520-21, Otsu, Japan aut NATIONALLICENCE 700 1- Ushijima T. Department of Microbiology, Shiga University of Medical Science, 520-21, Otsu, Japan aut NATIONALLICENCE 700 1- Niwa Y. Department of Dermatology, Kansai Medical University, 570, Moriguchi aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/7(1990-10-01), 449-454 0340-3696 282:7<449 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer