caa a22 4500 46577332X CHVBK 20180323111935.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00402619 doi (NATIONALLICENCE)springer-10.1007/BF00402619 Organ culture of human scalp hair follicles: effect of testosterone and oestrogen on hair growth [Elektronische Daten] [S. Kondo, Y. Hozumi, K. Aso] Summary: Whole human scalp hair follicles were cultured. The follicles were dissected from skin pieces of normal scalp and put into 1.5 ml of incubation medium in a closed 5 ml glass tube under an atmosphere of 95% O2 and 5% CO2. The tube was rolled at 15 rpm at 36‡C. Remarkable hair growth was noticed for 7 to 8 days. Hair root sheaths also grew with the hair shafts. The structure of the hair bulbs was well maintained for at least 6 days, and then the hair matrix cells started to degenerate. Fetal calf serum was not essential for hair growth in vitro, but increased the growth rate slowly. Testosterone and oestrogen inhibited hair growth in vitro to a similar extent. The minimum effective doses of both hormones to suppress hair growth were around 5 ng/ml, which corresponds well to the normal plasma level of testosterone in adult males in vivo, suggesting that scalp hair growth may be critically controlled by testosterone in adult males. Springer-Verlag, 1990 Organ culture nationallicence Human hair follicle nationallicence Hair growth nationallicence Testosterone nationallicence Oestrogen nationallicence Kondo S. Department of Dermatology, Yamagata University School of Medicine, 990-23, Yamagata City, Yamagata, Japan aut Hozumi Y. Department of Dermatology, Yamagata University School of Medicine, 990-23, Yamagata City, Yamagata, Japan aut Aso K. Department of Dermatology, Yamagata University School of Medicine, 990-23, Yamagata City, Yamagata, Japan aut Archives of Dermatological Research Springer-Verlag 282/7(1990-10-01), 442-445 0340-3696 282:7<442 1990 282 403 https://doi.org/10.1007/BF00402619 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00402619 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kondo S. Department of Dermatology, Yamagata University School of Medicine, 990-23, Yamagata City, Yamagata, Japan aut NATIONALLICENCE 700 1- Hozumi Y. Department of Dermatology, Yamagata University School of Medicine, 990-23, Yamagata City, Yamagata, Japan aut NATIONALLICENCE 700 1- Aso K. Department of Dermatology, Yamagata University School of Medicine, 990-23, Yamagata City, Yamagata, Japan aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/7(1990-10-01), 442-445 0340-3696 282:7<442 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer