caa a22 4500 465773338 CHVBK 20180323111935.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00372615 doi (NATIONALLICENCE)springer-10.1007/BF00372615 Binding of antibodies to nonhistone nucleoproteins on keratinocytes in suspensions [Elektronische Daten] [P. Velthuis, E. Wildschut, H. de la Faille, W. van Vloten, H. van Weelden, L. Kater] Summary: Antibody binding on the cell surface of epidermal cells, recently established on cultured neonatal foreskin cells, is supposed to play a role in the pathogenesis of in vivo antinuclear antibodies (ANA) of the skin. To study this phenomenon in suspensions of adult human keratinocytes, a cell system more closely related to the in vivo situation, we investigated the binding capacity of nine sera with various antibody profiles against nuclear components, as well as a murine monoclonal Sm-antibody. It was found that sera containing antibodies against nonhistone nucleoproteins bound to the cell surface of keratinocytes, whereas monospecific anti-dsDNA sera and the murine anti-Sm serum did not. This binding was found in both basal and suprabasal keratinocytes. The percentage of cells showing antibody binding was not significantly enhanced by preirradiation with ultraviolet light, as was found in a previously study. The cell surface binding is probably an antigen-antibody binding and not the result of cross-reactivity. Such cell surface binding may be important for the formation of in vivo ANA in the skin. Springer-Verlag, 1990 Lupus erythematosus nationallicence Photosensitivity nationallicence Antinuclear antibodies nationallicence dsDNA nationallicence Nonhistone nucleoproteins nationallicence Velthuis P. Department of Dermatology, University Hospital, Utrecht, The Netherlands aut Wildschut E. Department of Dermatology, University Hospital, Utrecht, The Netherlands aut de la Faille H. Department of Dermatology, University Hospital, Utrecht, The Netherlands aut van Vloten W. Department of Dermatology, University Hospital, Utrecht, The Netherlands aut van Weelden H. Department of Dermatology, University Hospital, Utrecht, The Netherlands aut Kater L. Division of Immunopathology, Department of Internal Medicine, University Hospital, Utrecht, The Netherlands aut Archives of Dermatological Research Springer-Verlag 282/3(1990-05-01), 159-163 0340-3696 282:3<159 1990 282 403 https://doi.org/10.1007/BF00372615 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00372615 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Velthuis P. Department of Dermatology, University Hospital, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Wildschut E. Department of Dermatology, University Hospital, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- de la Faille H. Department of Dermatology, University Hospital, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- van Vloten W. Department of Dermatology, University Hospital, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- van Weelden H. Department of Dermatology, University Hospital, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Kater L. Division of Immunopathology, Department of Internal Medicine, University Hospital, Utrecht, The Netherlands aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/3(1990-05-01), 159-163 0340-3696 282:3<159 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer