caa a22 4500 465773362 CHVBK 20180323111935.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00372620 doi (NATIONALLICENCE)springer-10.1007/BF00372620 Membrane attack complex of complement in Henoch-Schönlein purpura skin and nephritis [Elektronische Daten] [S. Kawana, G. Shen, Y. Kobayashi, S. Nishiyama] Summary: The present study using direct immunofluorescence with monoclonal antibodies to C5b-9 complex-related antigens was undertaken to determine whether complement activation in Henoch-Schönlein purpura (HSP) causes assembly of the membrane attack complex of complement (MAC) in skin and nephritis lesions. The deposition of C5, C6, C7, C8, C9, and C5b-9 neoantigens was noted in the vascular walls of papillary dermis and/or subpapillary dermal plexus of the vessels in 11 out of 15 patients with HSP. Their presence in vessel walls indicates complement activation which leads to terminal complement activation. There were small deposits of S protein at the same sites in three of the 11 skin specimens. Thus, the majority of C5b-9 demonstrated in HSP skin was the cytolytically active C5b-9 complex, MAC. Granular deposits of C5b-9 related antigens without S protein were also found in the capillary walls and mesangium of the glomeruli of two out of four specimens from patients with HSP nephritis; in the other two S protein was colocalized with the deposition of C5b-9. The results of the present study indicate that complement activation leading to generation of MAC may possibly be involved in the pathogenesis of vascular injury in a significantly large number of skin lesions and of HSP nephritis. Springer-Verlag, 1990 Henoch-Schönlein purpura nationallicence Complement nationallicence Cytolysis nationallicence Endothelial cell damage nationallicence Kawana S. Department of Dermatology, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, 228, Kanagawa, Japan aut Shen G. Department of Dermatology, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, 228, Kanagawa, Japan aut Kobayashi Y. Internal Medicine, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, 228, Kanagawa, Japan aut Nishiyama S. Department of Dermatology, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, 228, Kanagawa, Japan aut Archives of Dermatological Research Springer-Verlag 282/3(1990-05-01), 183-187 0340-3696 282:3<183 1990 282 403 https://doi.org/10.1007/BF00372620 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00372620 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kawana S. Department of Dermatology, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, 228, Kanagawa, Japan aut NATIONALLICENCE 700 1- Shen G. Department of Dermatology, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, 228, Kanagawa, Japan aut NATIONALLICENCE 700 1- Kobayashi Y. Internal Medicine, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, 228, Kanagawa, Japan aut NATIONALLICENCE 700 1- Nishiyama S. Department of Dermatology, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, 228, Kanagawa, Japan aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/3(1990-05-01), 183-187 0340-3696 282:3<183 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer