caa a22 4500 465773427 CHVBK 20180323111935.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00372614 doi (NATIONALLICENCE)springer-10.1007/BF00372614 Dexamethasone-induced plasminogen activator inhibitor: characterization, purification, and preparation of monoclonal antibodies [Elektronische Daten] [A. Oikarinen, M. HöyhtyÄ, M. JÄrvinen] Summary: The effects of dexamethasone on protein synthesis were studied in human fibrosarcoma (HT-1080) cells. Dexamethasone induced a new protein of 46 kD which was rapidly secreted into the medium, while neither progesterone nor estradiol would induce the synthesis of this protein and only a small increase in its amount could be seen in the presence of testosterone. The 46 kD protein was partially purified by ammonium sulfate precipitation and gel filtration and mouse monoclonal antibodies to it were produced in mouse hybrid cells. Altogether 13 positive clones were found, of which six reacted only with native and seven reacted with the unreduced 46 kD protein in Western blotting. It was possible by using polyclonal antibodies to plasminogen activator inhibitor type I (PAI-1) and purified plasminogen activator inhibitor type I to confirm that the 46 kD protein purified and characterized here was PAI-1. In addition, the 46 kD protein clearly inhibited plasminogen activation, thus further confirming that protein isolated was an inhibitor of plasminogen activator. Since the induction of PAI-1 by dexamethasone was very extensive, it is possible that glucocorticoids regulate proteolysis and fibrinolysis in vivo by increasing the amount of the inhibitor of plasminogen activator and thus preventing the activation of plasminogen to plasmin. The reduction of activation of plasminogen to plasmin by glucocorticoid-induced inhibitor could be of great importance, e.g., in various blistering diseases, in metastases from malignant cells, and in the migration of inflammatory cells. Springer-Verlag, 1990 Dexamethasone nationallicence Plasminogen nationallicence Monoclonal antibodies nationallicence Oikarinen A. Department of Dermatology, University of Oulu, SF-90220, Oulu, Finland aut HöyhtyÄ M. Department of Biochemistry, University of Oulu, SF-90220, Oulu, Finland aut JÄrvinen M. Department of Pathology, University of Oulu, SF-90220, Oulu, Finland aut Archives of Dermatological Research Springer-Verlag 282/3(1990-05-01), 153-158 0340-3696 282:3<153 1990 282 403 https://doi.org/10.1007/BF00372614 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00372614 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oikarinen A. Department of Dermatology, University of Oulu, SF-90220, Oulu, Finland aut NATIONALLICENCE 700 1- HöyhtyÄ M. Department of Biochemistry, University of Oulu, SF-90220, Oulu, Finland aut NATIONALLICENCE 700 1- JÄrvinen M. Department of Pathology, University of Oulu, SF-90220, Oulu, Finland aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/3(1990-05-01), 153-158 0340-3696 282:3<153 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer