caa a22 4500 465773451 CHVBK 20180323111935.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00372612 doi (NATIONALLICENCE)springer-10.1007/BF00372612 Common antigen expression between human periderm and other tissues identified by GB1-monoclonal antibody [Elektronische Daten] [O. Schofield, J. McDonald, D. Fredj-Reygrobellet, B. Hsi, C. Yeh, J. Ortonne, R. Eady] Summary: From 4 weeks estimated gestational age (EGA) until the end of the second trimester (24 weeks EGA) the fetal epidermis is covered by a specialised epithelium, the periderm. The origin and function of periderm remain speculative. We have demonstrated, using indirect immunofluorescence and immunoperoxidase staining, that periderm is recognised by a mouse IgM monoclonal antibody (Mab) GB1, which has been raised against a simple extract of human amnion. Immunoelectron microscopy localises GB1 to the amniotic surface of periderm, particularly in association with the microvilli, and also bordering cellular identations of the periderm cells. GB1 antigen (ag) is also expressed by the epithelium of fetal oesophagus, fetal and adult conjunctiva and cornea but is absent in a variety of other fetal and adult tissues including bladder, oral mucosa and thymus. The similar distribution of GB1 ag in both periderm and membranes possibly suggests a common origin and the shared expression with fetal oesophagus and fetal and adult eye may indicate a function related to the fluid environment. We therefore feel that GB1 Mab may be of use in further investigations into the origin, structure and function of human periderm. Springer-Verlag, 1990 GB1 monoclonal antibody nationallicence Periderm nationallicence Amnion nationallicence Schofield O. Department of Cell Pathology, Institute of Dermatology, St. Thomas' Hospital, SE1 7EH, London, UK aut McDonald J. Department of Cell Pathology, Institute of Dermatology, St. Thomas' Hospital, SE1 7EH, London, UK aut Fredj-Reygrobellet D. Département de Pharmacologie, Faculté de Médecine, Nice, France aut Hsi B. Faculté de Médecine, INSERM U210, Nice, France aut Yeh C. Faculté de Médecine, INSERM U210, Nice, France aut Ortonne J. Département de Dermatologie, Faculté de Médecine, Nice, France aut Eady R. Department of Cell Pathology, Institute of Dermatology, St. Thomas' Hospital, SE1 7EH, London, UK aut Archives of Dermatological Research Springer-Verlag 282/3(1990-05-01), 143-148 0340-3696 282:3<143 1990 282 403 https://doi.org/10.1007/BF00372612 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00372612 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schofield O. Department of Cell Pathology, Institute of Dermatology, St. Thomas' Hospital, SE1 7EH, London, UK aut NATIONALLICENCE 700 1- McDonald J. Department of Cell Pathology, Institute of Dermatology, St. Thomas' Hospital, SE1 7EH, London, UK aut NATIONALLICENCE 700 1- Fredj-Reygrobellet D. Département de Pharmacologie, Faculté de Médecine, Nice, France aut NATIONALLICENCE 700 1- Hsi B. Faculté de Médecine, INSERM U210, Nice, France aut NATIONALLICENCE 700 1- Yeh C. Faculté de Médecine, INSERM U210, Nice, France aut NATIONALLICENCE 700 1- Ortonne J. Département de Dermatologie, Faculté de Médecine, Nice, France aut NATIONALLICENCE 700 1- Eady R. Department of Cell Pathology, Institute of Dermatology, St. Thomas' Hospital, SE1 7EH, London, UK aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/3(1990-05-01), 143-148 0340-3696 282:3<143 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer