caa a22 4500 465773486 CHVBK 20180323111935.0 cr unu---uuuuu 170327e19900501xx s 000 0 eng 10.1007/BF00372621 doi (NATIONALLICENCE)springer-10.1007/BF00372621 Effect of genetically determined immunodeficiency on epidermal dendritic cell populations in C57BL/6J mice [Elektronische Daten] [E. Sprecher, Y. Becker, G. Kraal, E. Hall, L. Shultz] Summary: Mice homozygous for three different recessive mutations known to cause pleiotropic defects in the immune system and in the skin were used to evaluate the relationship between the classical immune system and dendritic epidermal cell populations. Numbers of Langerhans cells (LCs) and Thy-1+ dendritic cells (Thy-1+DEC) were determined using indirect immunofluorescence microscopy of epidermal whole mounts taken from viable motheaten (me v ), nude (nu), and rhino (rh hr ) mice. All mutants were maintained on the C57BL/6J strain background and were compared with their respective littermate normal controls. Viable motheaten mice had normal numbers of LCs at 1 month of age. However, by 8 weeks of age, LC density had decreased threefold. Nude and rhino mice had normal numbers of LCs at all ages tested. There was no significant effect of the viable motheaten mutation on numbers of Thy-1+DEC. Although nude mice showed normal numbers of Thy-1+ DEC at 1 month of age, these athymic mice had a threefold decrease in numbers of such cells by 6 months. In contrast to the reduced numbers of Thy-1+DEC seen in nude mice, rhino mice showed a four- to fivefold increase in the numbers of these epidermal cells at all ages tested. These findings suggest new mouse models for investigating the development, regulation, and biological properties of epidermal dendritic cell populations. Springer-Verlag, 1990 Immunodeficiency nationallicence Dendritic cells nationallicence Langerhans cells nationallicence Immunofluorescence microscopy nationallicence Sprecher E. Department of Molecular Virology, Faculty of Medicine, Hebrew University, Jerusalem, Israel aut Becker Y. Department of Molecular Virology, Faculty of Medicine, Hebrew University, Jerusalem, Israel aut Kraal G. Department of Histology, Free University, Amsterdam, The Netherlands aut Hall E. Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut Shultz L. Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut Archives of Dermatological Research Springer-Verlag 282/3(1990-05-01), 188-193 0340-3696 282:3<188 1990 282 403 https://doi.org/10.1007/BF00372621 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00372621 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sprecher E. Department of Molecular Virology, Faculty of Medicine, Hebrew University, Jerusalem, Israel aut NATIONALLICENCE 700 1- Becker Y. Department of Molecular Virology, Faculty of Medicine, Hebrew University, Jerusalem, Israel aut NATIONALLICENCE 700 1- Kraal G. Department of Histology, Free University, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Hall E. Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut NATIONALLICENCE 700 1- Shultz L. Jackson Laboratory, 04609, Bar Harbor, Maine, USA aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/3(1990-05-01), 188-193 0340-3696 282:3<188 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer