caa a22 4500 465773532 CHVBK 20180323111935.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00371647 doi (NATIONALLICENCE)springer-10.1007/BF00371647 Enhanced melanogenesis of murine melanoma cells cultured on or in collagen gel [Elektronische Daten] [T. Kono, M. Furukawa, T. Tanii, N. Mizuno, S. Taniguchi, M. Ishii, T. Hamada] Summary: To elucidate the interaction between melanoma and its matrix, we cultured B16 murine melanoma cells on and in type I collagen gel and evaluated specified functions of melanoma cells; tyrosinase activity and melanin-synthesizing capacity. Proliferation of cells cultured in these environments was markedly suppressed compared with that of cells cultured conventionally on plastic. On the other hand, the tyrosinase activity of cells cultured in or on collagen gel was two to three times higher than that of cells cultured on the plastics, while their melanin production was approximately double that achieved during conventional culture of cells. In conclusion, collagen gel influenced the growth and cell-specific functions of the melanoma cell. The culture system using collagen gel as substrate may be useful for the investigation of the interaction between melanoma and its matrix. Springer-Verlag, 1990 B16 melanoma cell nationallicence Collagen gel nationallicence Tyrosinase nationallicence Melanogenesis nationallicence Cell growth nationallicence Kono T. Department of Dermatology, Osaka City University Medical School, 1-5-7, Asahimachi, Abeno-ku, 545, Osaka, Japan aut Furukawa M. Department of Dermatology, Osaka City University Medical School, 1-5-7, Asahimachi, Abeno-ku, 545, Osaka, Japan aut Tanii T. Department of Dermatology, Osaka City University Medical School, 1-5-7, Asahimachi, Abeno-ku, 545, Osaka, Japan aut Mizuno N. Department of Dermatology, Osaka City University Medical School, 1-5-7, Asahimachi, Abeno-ku, 545, Osaka, Japan aut Taniguchi S. Department of Dermatology, Osaka City University Medical School, 1-5-7, Asahimachi, Abeno-ku, 545, Osaka, Japan aut Ishii M. Department of Dermatology, Osaka City University Medical School, 1-5-7, Asahimachi, Abeno-ku, 545, Osaka, Japan aut Hamada T. Department of Dermatology, Osaka City University Medical School, 1-5-7, Asahimachi, Abeno-ku, 545, Osaka, Japan aut Archives of Dermatological Research Springer-Verlag 282/4(1990-06-01), 263-266 0340-3696 282:4<263 1990 282 403 https://doi.org/10.1007/BF00371647 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00371647 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kono T. Department of Dermatology, Osaka City University Medical School, 1-5-7, Asahimachi, Abeno-ku, 545, Osaka, Japan aut NATIONALLICENCE 700 1- Furukawa M. Department of Dermatology, Osaka City University Medical School, 1-5-7, Asahimachi, Abeno-ku, 545, Osaka, Japan aut NATIONALLICENCE 700 1- Tanii T. Department of Dermatology, Osaka City University Medical School, 1-5-7, Asahimachi, Abeno-ku, 545, Osaka, Japan aut NATIONALLICENCE 700 1- Mizuno N. Department of Dermatology, Osaka City University Medical School, 1-5-7, Asahimachi, Abeno-ku, 545, Osaka, Japan aut NATIONALLICENCE 700 1- Taniguchi S. Department of Dermatology, Osaka City University Medical School, 1-5-7, Asahimachi, Abeno-ku, 545, Osaka, Japan aut NATIONALLICENCE 700 1- Ishii M. Department of Dermatology, Osaka City University Medical School, 1-5-7, Asahimachi, Abeno-ku, 545, Osaka, Japan aut NATIONALLICENCE 700 1- Hamada T. Department of Dermatology, Osaka City University Medical School, 1-5-7, Asahimachi, Abeno-ku, 545, Osaka, Japan aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/4(1990-06-01), 263-266 0340-3696 282:4<263 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer