caa a22 4500 465773591 CHVBK 20180323111935.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00371644 doi (NATIONALLICENCE)springer-10.1007/BF00371644 Effect of gamma-interferon on lectin-binding glycoproteins in cultured human keratinocytes [Elektronische Daten] [A. Reano, S. Hesse, J. Viac] Summary: We report the effect of exposure of human keratinocyte cultures to human recombinant gamma-interferon (g-IFN) on the expression of glycoproteins. Concanavalia ensiformis agglutinin (Con-A), and Arachis hypogaea agglutinin (PNA) were used to investigate expression of glycoproteins. NP-40 extracts from cultures grown with or without 100 U/ml g-IFN were analyzed by incubation of SDS-polyacrylamide gels with 125I-labeled lectins. Comparison of Con-A binding glycoprotein profiles showed both qualitative and quantitative changes related to the effect of g-IFN. Differences were also apparent after labeling of the gels with PNA. A limited number of components were labeled, with most of the reactivity falling within a couple of diffuse bands with high molecular weight (300 to 360 kDa). These components were strongly labeled in extracts from cells grown in the presence of g-IFN, but weakly reactive in control cultures. Neuraminidase treatment unmasked a 205 kDa PNA binding molecule only when cells were cultured in the absence of g-IFN. These changes are interpreted in terms of increased keratinocyte differentiation induced by g-IFN and demonstrate that glycoproteins bearing carbohydrate residues available to lectins Con-A and PNA have to be taken into account to better understand the complex action of this lymphokine. In inflammatory lesions, such changes in the glycoproteins of keratinocytes expressing HLA-DR antigens remain to be explored. Springer-Verlag, 1990 Epidermal cell cultures nationallicence Glycoproteins nationallicence Lectins nationallicence Gamma-interferon nationallicence Reano A. Laboratoire de Recherche Dermatologique et Immunologie, INSERM U. 209, CNRS URA. 0601, HÔpital E. Herriot, F-69437, Lyon Cedex 03, France aut Hesse S. Laboratoire de Recherche Dermatologique et Immunologie, INSERM U. 209, CNRS URA. 0601, HÔpital E. Herriot, F-69437, Lyon Cedex 03, France aut Viac J. Laboratoire de Recherche Dermatologique et Immunologie, INSERM U. 209, CNRS URA. 0601, HÔpital E. Herriot, F-69437, Lyon Cedex 03, France aut Archives of Dermatological Research Springer-Verlag 282/4(1990-06-01), 246-252 0340-3696 282:4<246 1990 282 403 https://doi.org/10.1007/BF00371644 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00371644 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reano A. Laboratoire de Recherche Dermatologique et Immunologie, INSERM U. 209, CNRS URA. 0601, HÔpital E. Herriot, F-69437, Lyon Cedex 03, France aut NATIONALLICENCE 700 1- Hesse S. Laboratoire de Recherche Dermatologique et Immunologie, INSERM U. 209, CNRS URA. 0601, HÔpital E. Herriot, F-69437, Lyon Cedex 03, France aut NATIONALLICENCE 700 1- Viac J. Laboratoire de Recherche Dermatologique et Immunologie, INSERM U. 209, CNRS URA. 0601, HÔpital E. Herriot, F-69437, Lyon Cedex 03, France aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/4(1990-06-01), 246-252 0340-3696 282:4<246 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer