caa a22 4500 465773605 CHVBK 20180323111935.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00371646 doi (NATIONALLICENCE)springer-10.1007/BF00371646 Cell cycle analysis of human dermal fibroblasts cultured on or in hydrated type I collagen lattices [Elektronische Daten] [T. Kono, T. Tanii, M. Furukawa, N. Mizuno, J. Kitajima, M. Ishii, T. Hamada, K. Yoshizato] Summary: The proliferation and cell cycle phase composition of human dermal fibroblasts cultured on or in type I collagen lattices (reconstituted dermis model) were examined. On collagen lattices, as compared with conventional cultures on plastic dishes, the proliferation of human dermal fibroblasts was suppressed, being arrested at about one-half the saturation density after 10 days of culture. In collagen lattices, proliferation was further suppressed, being nearly arrested within 4-7 days of culture. Cells were analyzed for cell cycle phases by two-color flow cytometry using DNA staining and S phase cell staining with FITC-conjugated antibromodeoxyuridine antibody. After 5 days of culture, the number of S phase cells on collagen lattices was 49.3% of that on plastic dishes, with an increase in G0G1 phase cells of 79.8%. In collagen lattices, the number of S phase cells was very small (4.3% of all cells), and most of the cells accumulated in G0G1 phase. These findings suggest that the cell cycle of fibroblasts is arrested at G0G1 phase by their interaction with collagen. On the basis of these results, the reconstituted dermis model using collagen lattice is considered to be analogous to the dermis in vivo with respect to cell growth and cell cycle phase composition. Springer-Verlag, 1990 Cllagen lattice nationallicence Fibroblast nationallicence Cell growth nationallicence Cell cycle nationallicence Flow cytometry nationallicence Kono T. Department of Dermatology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno-ku, 545, Osaka, Japan aut Tanii T. Department of Dermatology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno-ku, 545, Osaka, Japan aut Furukawa M. Department of Dermatology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno-ku, 545, Osaka, Japan aut Mizuno N. Department of Dermatology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno-ku, 545, Osaka, Japan aut Kitajima J. Department of Dermatology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno-ku, 545, Osaka, Japan aut Ishii M. Department of Dermatology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno-ku, 545, Osaka, Japan aut Hamada T. Department of Dermatology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno-ku, 545, Osaka, Japan aut Yoshizato K. Developmental Biology Laboratory, Zoological Institute, Faculty of Science, Hiroshima University, 1-1-89, Higashisenda-machi, Naka-ku, 730, Hiroshima, Japan aut Archives of Dermatological Research Springer-Verlag 282/4(1990-06-01), 258-262 0340-3696 282:4<258 1990 282 403 https://doi.org/10.1007/BF00371646 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00371646 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kono T. Department of Dermatology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno-ku, 545, Osaka, Japan aut NATIONALLICENCE 700 1- Tanii T. Department of Dermatology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno-ku, 545, Osaka, Japan aut NATIONALLICENCE 700 1- Furukawa M. Department of Dermatology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno-ku, 545, Osaka, Japan aut NATIONALLICENCE 700 1- Mizuno N. Department of Dermatology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno-ku, 545, Osaka, Japan aut NATIONALLICENCE 700 1- Kitajima J. Department of Dermatology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno-ku, 545, Osaka, Japan aut NATIONALLICENCE 700 1- Ishii M. Department of Dermatology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno-ku, 545, Osaka, Japan aut NATIONALLICENCE 700 1- Hamada T. Department of Dermatology, Osaka City University Medical School, 1-5-7 Asahimachi, Abeno-ku, 545, Osaka, Japan aut NATIONALLICENCE 700 1- Yoshizato K. Developmental Biology Laboratory, Zoological Institute, Faculty of Science, Hiroshima University, 1-1-89, Higashisenda-machi, Naka-ku, 730, Hiroshima, Japan aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/4(1990-06-01), 258-262 0340-3696 282:4<258 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer