caa a22 4500 46577363X CHVBK 20180323111935.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00371642 doi (NATIONALLICENCE)springer-10.1007/BF00371642 Cell shedding from human plantar skin in vitro: evidence that two different types of protein structures are degraded by a chymotrypsin-like enzyme [Elektronische Daten] [A. Lundström, T. Egelrud] Summary: A recently described endogenous proteolytic process in pieces of human plantar stratum corneum incubated in vitro has been further studied. This process leads to a decrease in cohesion between the cells that had been facing outwards in vivo. Using two methods, that differed with respect to efficiency, to detach surface cells with decreased cohesion, the process could be divided into two steps. The first step took place irrespective of the presence of ethylenediaminetetraacetate (EDTA) and led to a moderate decrease in cohesion between surface cells. The second step occurred only in the presence of EDTA and advanced to a point where the surface cells could be separated from the remaining cohesive tissue pieces by simple agitation. Both degradation steps could be inhibited by aprotinin and chymostatin but not by leupeptin. Zinc sulfate inhibited the first step. The results indicate that there are two different types of protein structures being degraded during the process of cell shedding in vitro. A chymotrypsin-like enzyme may be involved in the process. Springer-Verlag, 1990 Plantar stratum corneum nationallicence Cell shedding nationallicence Cohesive protein structures nationallicence Chymotrypsin-like enzyme nationallicence Lundström A. Department of Dermatology, University of Umeå, S-901 85, Umeå, Sweden aut Egelrud T. Department of Dermatology, University of Umeå, S-901 85, Umeå, Sweden aut Archives of Dermatological Research Springer-Verlag 282/4(1990-06-01), 234-237 0340-3696 282:4<234 1990 282 403 https://doi.org/10.1007/BF00371642 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00371642 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lundström A. Department of Dermatology, University of Umeå, S-901 85, Umeå, Sweden aut NATIONALLICENCE 700 1- Egelrud T. Department of Dermatology, University of Umeå, S-901 85, Umeå, Sweden aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/4(1990-06-01), 234-237 0340-3696 282:4<234 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer