caa a22 4500 465773672 CHVBK 20180323111936.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00371942 doi (NATIONALLICENCE)springer-10.1007/BF00371942 Detection of human papillomavirus (HPV) DNA in oral squamous cell carcinomas by in situ hybridization and polymerase chain reaction [Elektronische Daten] [Fuju Chang, S. SyrjÄnen, J. Nuutinen, J. KÄrjÄ, K. SyrjÄnen] Summary: A series of 156 formalin-fixed, paraffin-embedded biopsies from 40 patients with surgically-treated oral squamous cell carcinomas was analysed for the presence of human papillomavirus (HPV) infection by histopathological evaluation, in situ DNA hybridization and polymerase chain reaction (PCR). Epithelial changes suggesting a HPV lesion within, or adjacent to, the carcinoma lesions were found in 16 out of 40 patients (40%). Morphological signs of a flat HPV lesion were found in four cases (10%), those of inverted type in three cases (7.5%), and those of papillary type in nine cases (22.5%). HPV DNA was demonstrated in one of the lesions by in situ hybridization with biotin-labelled DNA cocktail probe containing HPV types 6, 11, 16 and 18. With the PCR technique, samples from 11 (27.5%) of the 40 patients proved to contain HPV DNA. Of these, HPV 6 was demonstrated in one case, HPV 16 in ten cases and HPV 18 in one case. HPV DNA was exclusively detected in the biopsies showing carcinoma tissue or its adjacent precancer lesions. No viral DNA was found in the biopsies derived from the tumour-free resection margins. These results provide further evidence to support the concept of HPV involvement in the aetiology of oral squamous cell carcinomas, most probably acting synergistically with other carcinogens. Springer-Verlag, 1990 Oral squamous cell carcinoma nationallicence Human papillomavirus (HPV) nationallicence Polymerase chain reaction (PCR) nationallicence Carcinoma lesions nationallicence Chang Fuju Department of Pathology, University of Kuopio, Kuopio, Finland aut SyrjÄnen S. Kuopio Cancer Research Centre, University of Kuopio, Kuopio, Finland aut Nuutinen J. Department of Otolaryngology, University of Kuopio, Kuopio, Finland aut KÄrjÄ J. Department of Otolaryngology, University of Kuopio, Kuopio, Finland aut SyrjÄnen K. Department of Pathology, University of Kuopio, Kuopio, Finland aut Archives of Dermatological Research Springer-Verlag 282/8(1990-12-01), 493-497 0340-3696 282:8<493 1990 282 403 https://doi.org/10.1007/BF00371942 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00371942 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chang Fuju Department of Pathology, University of Kuopio, Kuopio, Finland aut NATIONALLICENCE 700 1- SyrjÄnen S. Kuopio Cancer Research Centre, University of Kuopio, Kuopio, Finland aut NATIONALLICENCE 700 1- Nuutinen J. Department of Otolaryngology, University of Kuopio, Kuopio, Finland aut NATIONALLICENCE 700 1- KÄrjÄ J. Department of Otolaryngology, University of Kuopio, Kuopio, Finland aut NATIONALLICENCE 700 1- SyrjÄnen K. Department of Pathology, University of Kuopio, Kuopio, Finland aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/8(1990-12-01), 493-497 0340-3696 282:8<493 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer