caa a22 4500 465773702 CHVBK 20180323111936.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00371943 doi (NATIONALLICENCE)springer-10.1007/BF00371943 In vivo percutaneous absorption: a key role for stratum corneum/vehicle partitioning [Elektronische Daten] [A. Rougier, M. Rallis, P. Krien, C. Lotte] Summary: Percutaneous absorption of five compounds was studied in the hairless rat in vivo: benzoic acid, caffeine, hydrocortisone, inulin and thiourea. The results clearly demonstrate that, as with in vitro experiments, a steady-state flux can be achieved in vivo. This steady-state flux is strongly molecule dependent. Thus, the values for inulin and benzoic acid differ by a factor of about 40. In contrast, although the physicochemical properties of the studied compounds vary widely, their lag times were not significantly different. The mean lag time was 11±2 min. Different compounds could be considered to have approximately the same apparent diffusion coefficient with regard to their percutaneous absorption in vivo. Thus, for a given thickness of stratum corneum and a given anatomical site, the penetration flux value of a substance depends only on its stratum corneum/vehicle partition coefficient. Using a classical model, we have demonstrated that the amount of substance present in the stratum corneum (Q sc) at equilibrium (30 min) is related to this partition coefficient. There is also a linear relationship between steady-state flux and Q sc. In practice, the in vivo steady-state flux of penetration of a compound can be predicted from the simple measurement of the amount present in the stratum corneum after a contact time of 30 min. Springer-Verlag, 1990 Percutaneous absorption nationallicence Steady-state flux nationallicence Stratum corneum nationallicence Vehicle nationallicence Partition coefficient nationallicence Rougier A. Départment de Biologie, Centre de Recherche Fondamentale de l'Oreal, 1, avenue E Schueller, F-93601, Aulnay sous Bois, France aut Rallis M. Départment de Biologie, Centre de Recherche Fondamentale de l'Oreal, 1, avenue E Schueller, F-93601, Aulnay sous Bois, France aut Krien P. Départment de Biologie, Centre de Recherche Fondamentale de l'Oreal, 1, avenue E Schueller, F-93601, Aulnay sous Bois, France aut Lotte C. Départment de Biologie, Centre de Recherche Fondamentale de l'Oreal, 1, avenue E Schueller, F-93601, Aulnay sous Bois, France aut Archives of Dermatological Research Springer-Verlag 282/8(1990-12-01), 498-505 0340-3696 282:8<498 1990 282 403 https://doi.org/10.1007/BF00371943 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00371943 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rougier A. Départment de Biologie, Centre de Recherche Fondamentale de l'Oreal, 1, avenue E Schueller, F-93601, Aulnay sous Bois, France aut NATIONALLICENCE 700 1- Rallis M. Départment de Biologie, Centre de Recherche Fondamentale de l'Oreal, 1, avenue E Schueller, F-93601, Aulnay sous Bois, France aut NATIONALLICENCE 700 1- Krien P. Départment de Biologie, Centre de Recherche Fondamentale de l'Oreal, 1, avenue E Schueller, F-93601, Aulnay sous Bois, France aut NATIONALLICENCE 700 1- Lotte C. Départment de Biologie, Centre de Recherche Fondamentale de l'Oreal, 1, avenue E Schueller, F-93601, Aulnay sous Bois, France aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/8(1990-12-01), 498-505 0340-3696 282:8<498 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer