caa a22 4500 465773753 CHVBK 20180323111936.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00371945 doi (NATIONALLICENCE)springer-10.1007/BF00371945 Vimentin expression in normal human keratinocytes grown in serum-free defined MCDB 153 medium [Elektronische Daten] [M. Richard, J. Viac, A. Reano, M. Gaucherand, J. Thivolet] Summary: We have shown that subcultured keratinocytes derived from normal human skin and grown on plastic in serum-free defined medium (MCDB 153, with 0.1 mM Ca2+ concentration) synthesize vimentin. A fibrillar vimentin expression was observed in the cell cytoplasm by immunohistochemistry with different monoclonal antibodies to vimentin in a high proportion of cultured cells. Two dimensional gel electrophoresis of cytoskeletal proteins from keratinocyte subcultures and immunoblotting reaction with different monoclonal antibodies to vimentin showed a specific reaction in a position corresponding to vimentin. No cross-reaction with keratin polypeptides was obtained. This expression of vimentin may be related to the adaptation of cells to in vitro growth conditions. Springer-Verlag, 1990 Human keratinocyte culture nationallicence Keratin nationallicence Vimentin nationallicence Immunodetection nationallicence Richard M. Laboratoire de Recherche Dermatologique et Immunologie, INSERM U. 209, CNRS URA 601, HÔpital E. Herriot, F-69437, Lyon, Cedex 03, France aut Viac J. Laboratoire de Recherche Dermatologique et Immunologie, INSERM U. 209, CNRS URA 601, HÔpital E. Herriot, F-69437, Lyon, Cedex 03, France aut Reano A. Laboratoire de Recherche Dermatologique et Immunologie, INSERM U. 209, CNRS URA 601, HÔpital E. Herriot, F-69437, Lyon, Cedex 03, France aut Gaucherand M. Laboratoire de Recherche Dermatologique et Immunologie, INSERM U. 209, CNRS URA 601, HÔpital E. Herriot, F-69437, Lyon, Cedex 03, France aut Thivolet J. Laboratoire de Recherche Dermatologique et Immunologie, INSERM U. 209, CNRS URA 601, HÔpital E. Herriot, F-69437, Lyon, Cedex 03, France aut Archives of Dermatological Research Springer-Verlag 282/8(1990-12-01), 512-515 0340-3696 282:8<512 1990 282 403 https://doi.org/10.1007/BF00371945 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00371945 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Richard M. Laboratoire de Recherche Dermatologique et Immunologie, INSERM U. 209, CNRS URA 601, HÔpital E. Herriot, F-69437, Lyon, Cedex 03, France aut NATIONALLICENCE 700 1- Viac J. Laboratoire de Recherche Dermatologique et Immunologie, INSERM U. 209, CNRS URA 601, HÔpital E. Herriot, F-69437, Lyon, Cedex 03, France aut NATIONALLICENCE 700 1- Reano A. Laboratoire de Recherche Dermatologique et Immunologie, INSERM U. 209, CNRS URA 601, HÔpital E. Herriot, F-69437, Lyon, Cedex 03, France aut NATIONALLICENCE 700 1- Gaucherand M. Laboratoire de Recherche Dermatologique et Immunologie, INSERM U. 209, CNRS URA 601, HÔpital E. Herriot, F-69437, Lyon, Cedex 03, France aut NATIONALLICENCE 700 1- Thivolet J. Laboratoire de Recherche Dermatologique et Immunologie, INSERM U. 209, CNRS URA 601, HÔpital E. Herriot, F-69437, Lyon, Cedex 03, France aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/8(1990-12-01), 512-515 0340-3696 282:8<512 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer