caa a22 4500 465773788 CHVBK 20180323111936.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00375729 doi (NATIONALLICENCE)springer-10.1007/BF00375729 Schill W. Department of Dermatology and Andrology, Justus Liebig University, Gaffkystrasse 14, D-6300, Giessen, Germany aut Determination of active, non-zymogen acrosin, proacrosin and total acrosin in different andrological patients [Elektronische Daten] [W. Schill] Summary: A spectrophotometric assay is described which, due to improved extraction conditions, allows quantitative determination of enzymatically active, non-zymogen acrosin, proacrosin and total acrosin activity from human sperm acrosomes. Acrosomal proteinase activity is assessed by acid extraction of the sperm pellet and the suspension medium before and after snap-freezing, followed by zymogen autoactivation. Release of acrosin from the acrosome can be used as a sensitive biochemical marker to characterize acrosomal membrane stability, severe disturbance of which may be the cause of impaired male fertility. Acrosin activities in different populations of semen specimens are reported and compared to data available in the literature. Different degrees of acrosomal membrane alterations are observed in men with oligozoospermia, teratozoospermia and polyzoospermia. Particularly in oligozoospermia, a significant increase of active, nonzymogen acrosin points to severe acrosomal membrane alterations and, in addition, to a premature activation of proacrosin, which may impair fertilization in certain individuals. Finally, acrosin activity is shown to be significantly influenced by the time of sexual abstinence. It is concluded that determination of acrosin may be a useful indicator of the fertility potential in men. Springer-Verlag, 1990 Acrosin active, non-zymogen nationallicence Proacrosin nationallicence Acrosomal membrane stability/disturbances nationallicence Fertility/sterility nationallicence Archives of Dermatological Research Springer-Verlag 282/5(1990-08-01), 335-342 0340-3696 282:5<335 1990 282 403 https://doi.org/10.1007/BF00375729 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00375729 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Schill W. Department of Dermatology and Andrology, Justus Liebig University, Gaffkystrasse 14, D-6300, Giessen, Germany aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/5(1990-08-01), 335-342 0340-3696 282:5<335 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer