caa a22 4500 465773842 CHVBK 20180323111936.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00375725 doi (NATIONALLICENCE)springer-10.1007/BF00375725 Immunohistological evaluation of basal cell carcinoma immunoinfiltrate during intralesional treatment with alpha2-interferon [Elektronische Daten] [N. Mozzanica, A. Cattaneo, V. Boneschi, L. Brambilla, E. Melotti, A. Finzi] Summary: We investigated the peritumoral and intratumoral immune infiltrate in 6 basal cell carcinomas (BCCs) treated with recombinant alpha2b-interferon. Each BCC was injected intralesionally three times a week for 3 weeks with 1.5×106 IU of interferon per injection (total dose 13.5×106 IU). The immunohistological study was done before the start of interferon therapy and 15 days afterwards, using a series of monoclonal antibodies and an immunocytochemical technique. Before therapy the infiltrate consisted mainly of CD3+ (T) cells, with prevalence of CD4+ (helper/inducer) T cells. The percentage of T cells expressing interleukin-2 receptor (CD25+ cells) was higher in the tumor nests than in the peritumoral infiltrate (20% and 11% respectively). CD1+ (Langerhans) cells and CD14b+ cells (monocytes/macrophages) were present in the peritumoral infiltrate in all cases (9%±5% and 14%±7% respectively). Very few CD56+ (natural killer), CD15+ (granulocytes) and CD20+ (B) cells were observed in the peritumoral infiltrate and none at all in tumor nests. After 15 days of interferon therapy, we observed an increase in peritumoral and intratumoral CD4+ cells. There was a decrease in the number of CD25+ cells and of CD1+ cells in the peritumoral infiltrate. The number of intratumoral CD25+ increased. No variations were seen in CD14b, CD15, CD20, and CD56 positive cells. Eight weeks after completion of therapy, two BCCs were cleared and the remaining four showed clinical and histological improvement. These results may indicate a direct effect of interferon against BCC; in addition the immunohistological findings suggest that intralesional interferon enhances T cell mediated immune response, especially in tumor nests. Interferon may therefore act against BCC as a cytotoxic agent and as an immunomodulator. Springer-Verlag, 1990 Basal cell carcinoma nationallicence Alpha2-interferon nationallicence Immunohistology nationallicence Mozzanica N. Second Department of Dermatology, University of Milan, Via Pace 9, I-20122, Milan, Italy aut Cattaneo A. Second Department of Dermatology, University of Milan, Via Pace 9, I-20122, Milan, Italy aut Boneschi V. Second Department of Dermatology, University of Milan, Via Pace 9, I-20122, Milan, Italy aut Brambilla L. Second Department of Dermatology, University of Milan, Via Pace 9, I-20122, Milan, Italy aut Melotti E. Second Department of Dermatology, University of Milan, Via Pace 9, I-20122, Milan, Italy aut Finzi A. Second Department of Dermatology, University of Milan, Via Pace 9, I-20122, Milan, Italy aut Archives of Dermatological Research Springer-Verlag 282/5(1990-08-01), 311-317 0340-3696 282:5<311 1990 282 403 https://doi.org/10.1007/BF00375725 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00375725 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mozzanica N. Second Department of Dermatology, University of Milan, Via Pace 9, I-20122, Milan, Italy aut NATIONALLICENCE 700 1- Cattaneo A. Second Department of Dermatology, University of Milan, Via Pace 9, I-20122, Milan, Italy aut NATIONALLICENCE 700 1- Boneschi V. Second Department of Dermatology, University of Milan, Via Pace 9, I-20122, Milan, Italy aut NATIONALLICENCE 700 1- Brambilla L. Second Department of Dermatology, University of Milan, Via Pace 9, I-20122, Milan, Italy aut NATIONALLICENCE 700 1- Melotti E. Second Department of Dermatology, University of Milan, Via Pace 9, I-20122, Milan, Italy aut NATIONALLICENCE 700 1- Finzi A. Second Department of Dermatology, University of Milan, Via Pace 9, I-20122, Milan, Italy aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/5(1990-08-01), 311-317 0340-3696 282:5<311 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer