caa a22 4500 465773869 CHVBK 20180323111936.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00375723 doi (NATIONALLICENCE)springer-10.1007/BF00375723 Properties of solubilized Fcγ-receptors from psoriatic scales [Elektronische Daten] [J. Livden, E. Kristoffersen, R. Matre] Summary: Extracts from psoriatic scales, prepared using Tris-HCl buffer containing ethylenediaminetetraacetatic acid (EDTA) and 2-mercaptoethanol (ME), agglutinated erythrocytes (E) sensitized with IgG antibodies (A) (EA), but not E or E sensitized with F(ab′)2-fragments of IgG. The agglutination was inhibited by IgG and Fc fragments of IgG, but not by IgA, IgM or F(ab′)2-fragments of IgG. Partially reduced and alkylated IgG did not inhibit the agglutination, indicating that an inter-heavy-chain disulphide-linked Fc region is required for binding of FcR. The extracts inhibited EA, but not E or EAC rosette formation with mononuclear cells. The results strongly indicated that the extract contained functionally active FcR. The agglutinating activity of the extract was not affected by treatment with periodic acid or formaldehyde, whereas heat reduced the activity. Using a monoclonal antibody (B1D6) a functionally active 40 kDa FcR with low affinity for native IgG was purified from the scale extract. The extracts also contained FcR activity not recognized by B1D6. Springer-Verlag, 1990 Psoriatic scale extract nationallicence Fcγ-receptors nationallicence Monoclonal antibody nationallicence IgG purification nationallicence Livden J. Department of Dermatology, University of Bergen, Bergen, Norway aut Kristoffersen E. Broegelmann Research Laboratory for Microbiology, University of Bergen, Bergen, Norway aut Matre R. Broegelmann Research Laboratory for Microbiology, University of Bergen, Bergen, Norway aut Archives of Dermatological Research Springer-Verlag 282/5(1990-08-01), 300-303 0340-3696 282:5<300 1990 282 403 https://doi.org/10.1007/BF00375723 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00375723 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Livden J. Department of Dermatology, University of Bergen, Bergen, Norway aut NATIONALLICENCE 700 1- Kristoffersen E. Broegelmann Research Laboratory for Microbiology, University of Bergen, Bergen, Norway aut NATIONALLICENCE 700 1- Matre R. Broegelmann Research Laboratory for Microbiology, University of Bergen, Bergen, Norway aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/5(1990-08-01), 300-303 0340-3696 282:5<300 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer