caa a22 4500 465773885 CHVBK 20180323111936.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00375728 doi (NATIONALLICENCE)springer-10.1007/BF00375728 Growth advantage by overexpression of normal Harvey ras proto-oncogene in cultured rat epidermal keratinocytes [Elektronische Daten] [K. Yamanishi, F. Liew, Y. Hosokawa, S. Kishimoto, H. Yasuno] Summary: The ras proto-oncogene is frequently amplified and overexpressed in the hyperproliferative conditions of epidermal keratinocytes. To investigate the effects of its overexpression on the growth of keratinocytes in a model system, we constructed expression vectors for normal human Ha-ras and introduced them into FRSK cells, a fetal rat epidermal keratinocyte cell line. Several clones containing the transfected Ha-ras were isolated, and two of them overexpressed this gene. In these clones DNA synthesis and cell growth were greater than in other clones expressing this gene at low levels. Thus we suggest that overexpression of normal ras gene may provide growth advantage to epidermal keratinocytes. Springer-Verlag, 1990 Harvey ras proto-oncogene nationallicence Gene expression nationallicence Keratinocytes nationallicence Cell growth control nationallicence Yamanishi K. Department of Dermatology, Kyoto Prefectural University of Medicine, Kamigyo-ku, 602, Kyoto, Japan aut Liew F. Department of Dermatology, Kyoto Prefectural University of Medicine, Kamigyo-ku, 602, Kyoto, Japan aut Hosokawa Y. Department of Preventive Medicine, Kyoto Prefectural University of Medicine, Kamigyo-ku, 602, Kyoto, Japan aut Kishimoto S. Department of Dermatology, Kyoto Prefectural University of Medicine, Kamigyo-ku, 602, Kyoto, Japan aut Yasuno H. Department of Dermatology, Kyoto Prefectural University of Medicine, Kamigyo-ku, 602, Kyoto, Japan aut Archives of Dermatological Research Springer-Verlag 282/5(1990-08-01), 330-334 0340-3696 282:5<330 1990 282 403 https://doi.org/10.1007/BF00375728 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00375728 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamanishi K. Department of Dermatology, Kyoto Prefectural University of Medicine, Kamigyo-ku, 602, Kyoto, Japan aut NATIONALLICENCE 700 1- Liew F. Department of Dermatology, Kyoto Prefectural University of Medicine, Kamigyo-ku, 602, Kyoto, Japan aut NATIONALLICENCE 700 1- Hosokawa Y. Department of Preventive Medicine, Kyoto Prefectural University of Medicine, Kamigyo-ku, 602, Kyoto, Japan aut NATIONALLICENCE 700 1- Kishimoto S. Department of Dermatology, Kyoto Prefectural University of Medicine, Kamigyo-ku, 602, Kyoto, Japan aut NATIONALLICENCE 700 1- Yasuno H. Department of Dermatology, Kyoto Prefectural University of Medicine, Kamigyo-ku, 602, Kyoto, Japan aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/5(1990-08-01), 330-334 0340-3696 282:5<330 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer