caa a22 4500 465773915 CHVBK 20180323111936.0 cr unu---uuuuu 170327e19900801xx s 000 0 eng 10.1007/BF00375726 doi (NATIONALLICENCE)springer-10.1007/BF00375726 Effects of interferon-γ treatment on the cutaneous DTH reaction in rats [Elektronische Daten] [C. Skoglund, A. Scheynius] Summary: The capacity of interferon-γ (IFN-γ) to induce class II histocompatibility antigens on different cell types including keratinocytes, is well known, but the impact of IFN-γ on the immune response is still unclear. Lewis rats sensitized with dinitrofluorobenzene (DNFB) were injected with recombinant rat IFN-γ (105 U) or phosphate-buffered saline (PBS) once daily on 3 successive days at the bases of the ears either before or after they were challenged on the ears. As expected, the PBS-treated animals showed about a 30% increase in ear thickness and there was an induced expression of class II antigens on the keratinocytes as judged by immunohistochemistry 72 h after challenge. Exogenously added IFN-γ prior to DNFB challenge resulted in a significantly reduced ear swelling at 24 (p<0.01) and 48 h (p<0.05) after challenge. In this case the keratinocytes expressed class II antigens already at the time of challenge. When IFN-γ injections were given during the contact allergic reaction there was no significant reduction of ear swelling until 72 h (p<0.01). At that time point there was a more pronounced expression of class II antigens on the keratinocytes compared with PBS-injected animals, due to the IFN-γ treatment. These in vivo data support our previous observations that IFN-γ may play a self-limiting role in certain immune responses. Springer-Verlag, 1990 Class II antigens nationallicence Contact allergic reaction nationallicence Interferon-gamma nationallicence Rat skin nationallicence Skoglund C. Department of Clinical Immunology, Karolinska Institute, S-104 01, Stockholm, Sweden aut Scheynius A. Department of Clinical Immunology, Karolinska Institute, S-104 01, Stockholm, Sweden aut Archives of Dermatological Research Springer-Verlag 282/5(1990-08-01), 318-324 0340-3696 282:5<318 1990 282 403 https://doi.org/10.1007/BF00375726 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00375726 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Skoglund C. Department of Clinical Immunology, Karolinska Institute, S-104 01, Stockholm, Sweden aut NATIONALLICENCE 700 1- Scheynius A. Department of Clinical Immunology, Karolinska Institute, S-104 01, Stockholm, Sweden aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/5(1990-08-01), 318-324 0340-3696 282:5<318 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer