caa a22 4500 465773931 CHVBK 20180323111936.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00493462 doi (NATIONALLICENCE)springer-10.1007/BF00493462 Antikinetochore and antitopoisomerase I antibodies in systemic scleroderma: comparative study using immunoblotted recombinant antigens, immunofluorescence, and double immunodiffusion [Elektronische Daten] [M. Jarzabek-Chorzelska, M. Blaszczyk, Z. Kolacinska-Strasz, T. Chorzelski, S. Jabłońska, G. Maul] Summary: In 135 patients with systemic scleroderma, we compared three different methods to determine antinuclear autoantibody (ANA) specificity: indirect immunofluorescence, double immunodiffusion, and, employing recombinant antigens, immunoblotting using both marker autoantigens of this disease. A characteristic Scl-70 antibody pattern was found on HEp-2 cells in 83.8% of the patients, double immunodiffusion was positive for the Scl-70 antibodies in 81.9%, and immunoblot with the recombinant topoisomerase I (Topo I) was positive in 71% of the patients. For the centromere autoantibodies we found a high concordance between the anticentromere antibody (ACA) pattern on HEp-2 cells (27 patients positive) and the detection of recombinant kinetochore in immunoblotting (26 patients positive). The three testing techniques gave comparable results, except that the Topo I recombinant antigen used in immunoblotting reacted strongly with fewer than expected of the known Scl-70-positive sera. However, a method using recombinant antigens expressing all epitopes (rather than one of the epitopes of Topo I) will undoubtedly become the method of choice for detecting antibodies in systemic scleroderma. Using the immunoblotting technique with the recombinant antigens we detected in four patients antibodies against both Topo I and kinetochore. More severe symptoms of systemic scleroderma were found in patients who had both antibodies. The combined presence of both marker autoantibodies is therefore not as rare as previously reported and may predict severe disease. Springer-Verlag, 1990 Anticentromere antibodies in scleroderma nationallicence Antikinetochore antibodies in scleroderma nationallicence Topoisomerase I in scleroderma nationallicence Immunofluorescence nationallicence Immunodiffusion nationallicence Immunoblotting nationallicence Jarzabek-Chorzelska M. Department of Dermatology, Warsaw School of Medicine, Koszykowa 82a, PL-02-008, Warszawa, Poland aut Blaszczyk M. Department of Dermatology, Warsaw School of Medicine, Koszykowa 82a, PL-02-008, Warszawa, Poland aut Kolacinska-Strasz Z. Department of Dermatology, Warsaw School of Medicine, Koszykowa 82a, PL-02-008, Warszawa, Poland aut Chorzelski T. Department of Dermatology, Warsaw School of Medicine, Koszykowa 82a, PL-02-008, Warszawa, Poland aut Jabłońska S. Department of Dermatology, Warsaw School of Medicine, Koszykowa 82a, PL-02-008, Warszawa, Poland aut Maul G. Wistar Institute of Anatomy and Biology, Philadelphia, PA, USA aut Archives of Dermatological Research Springer-Verlag 282/2(1990-01-01), 76-83 0340-3696 282:2<76 1990 282 403 https://doi.org/10.1007/BF00493462 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00493462 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jarzabek-Chorzelska M. Department of Dermatology, Warsaw School of Medicine, Koszykowa 82a, PL-02-008, Warszawa, Poland aut NATIONALLICENCE 700 1- Blaszczyk M. Department of Dermatology, Warsaw School of Medicine, Koszykowa 82a, PL-02-008, Warszawa, Poland aut NATIONALLICENCE 700 1- Kolacinska-Strasz Z. Department of Dermatology, Warsaw School of Medicine, Koszykowa 82a, PL-02-008, Warszawa, Poland aut NATIONALLICENCE 700 1- Chorzelski T. Department of Dermatology, Warsaw School of Medicine, Koszykowa 82a, PL-02-008, Warszawa, Poland aut NATIONALLICENCE 700 1- Jabłońska S. Department of Dermatology, Warsaw School of Medicine, Koszykowa 82a, PL-02-008, Warszawa, Poland aut NATIONALLICENCE 700 1- Maul G. Wistar Institute of Anatomy and Biology, Philadelphia, PA, USA aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/2(1990-01-01), 76-83 0340-3696 282:2<76 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer