caa a22 4500 465773958 CHVBK 20180323111936.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00493471 doi (NATIONALLICENCE)springer-10.1007/BF00493471 Flow cytometric quantification of human epidermal cells expressing keratin 16 in vivo after standardized trauma [Elektronische Daten] [S. de Mare, P. van Erp, F. Ramaekers, P. van de Kerkhof] Summary: The intermediate filament protein keratin 16 is expressed in hyperproliferative epidermis. The present study aims to clarify the relationship between the expression of this keratin type, hyperproliferation (percentage of cells in SG2M phases), and keratinization (keratin 10 expression). These three parameters were quantified in biopsy material taken at different time intervals following sellotape stripping — this being a dynamic in vivo model for the induction of hyperproliferation. From the biopsy specimens cell suspensions were prepared, labeled with antibodies Ks8.12 (specially directed against keratin 16) and RKSE60 (directed against keratin 10), and analyzed using flow cytometry. Percentages of cells in SG2M phases were assessed by measuring the relative DNA content after propidium iodide staining. Keratin 16 expression in the suprabasal layer anticipated epidermal proliferation, suggesting a role of the suprabasal compartment in the induction of epidermal growth. Keratin 10 expression decreased about 1 day after the onset of keratin 16 expression, indicating that these processes do not depend directly upon each other. Springer-Verlag, 1990 Hyperproliferation nationallicence Keratin nationallicence Human epidermis nationallicence Flow cytometry nationallicence Monoclonal antibody nationallicence de Mare S. Department of Dermatology, University Hospital, Javastraat 104, NL-6524, MJ Nijmegen, The Netherlands aut van Erp P. Department of Dermatology, University Hospital, Javastraat 104, NL-6524, MJ Nijmegen, The Netherlands aut Ramaekers F. Department of Pathology, University Hospital, Javastraat 104, NL-6524, MJ Nijmegen, The Netherlands aut van de Kerkhof P. Department of Dermatology, University Hospital, Javastraat 104, NL-6524, MJ Nijmegen, The Netherlands aut Archives of Dermatological Research Springer-Verlag 282/2(1990-01-01), 126-130 0340-3696 282:2<126 1990 282 403 https://doi.org/10.1007/BF00493471 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00493471 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Mare S. Department of Dermatology, University Hospital, Javastraat 104, NL-6524, MJ Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- van Erp P. Department of Dermatology, University Hospital, Javastraat 104, NL-6524, MJ Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Ramaekers F. Department of Pathology, University Hospital, Javastraat 104, NL-6524, MJ Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- van de Kerkhof P. Department of Dermatology, University Hospital, Javastraat 104, NL-6524, MJ Nijmegen, The Netherlands aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/2(1990-01-01), 126-130 0340-3696 282:2<126 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer