caa a22 4500 465773974 CHVBK 20180323111936.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00493469 doi (NATIONALLICENCE)springer-10.1007/BF00493469 Histamine H1-receptor of vascular endothelial cells cultured from guinea pig skin [Elektronische Daten] [N. Noso, M. Hide, K. Nakamura, S. Yamamoto] Summary: In this study, a new procedure for the cultivation of dermal vascular endothelial cells from guinea pig ear lobe was established, and specific binding of [3H]mepyramine to the cultured cell membranes was demonstrated. The vascular endothelial cells were successfully isolated from the dermis of guinea pig ear lobes by the intravenous injection of dispase. Guinea pig serum, as a supplementary component in culture medium, is more suitable for the culture of guinea pig dermal vascular endothelial cell than is fetal calf serum. The [3H]mepyramine binding study indicated the presence of a large number of H1-receptors on the membrane of dermal vascular endothelial cells of guinea pigs, suggesting the important role of endothelial cells in cutaneous reactions to histamine. Springer-Verlag, 1990 Histamine H1-receptor nationallicence [3H]Mepyramine binding nationallicence Vascular endothelial cell nationallicence Guinea pig skin nationallicence Noso N. Department of Dermatology, Hiroshima University, School of Medicine, 1-2-3, Kasumi, Minami-ku, 734, Hiroshima, Japan aut Hide M. Department of Dermatology, Hiroshima University, School of Medicine, 1-2-3, Kasumi, Minami-ku, 734, Hiroshima, Japan aut Nakamura K. Department of Dermatology, Hiroshima University, School of Medicine, 1-2-3, Kasumi, Minami-ku, 734, Hiroshima, Japan aut Yamamoto S. Department of Dermatology, Hiroshima University, School of Medicine, 1-2-3, Kasumi, Minami-ku, 734, Hiroshima, Japan aut Archives of Dermatological Research Springer-Verlag 282/2(1990-01-01), 115-119 0340-3696 282:2<115 1990 282 403 https://doi.org/10.1007/BF00493469 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00493469 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Noso N. Department of Dermatology, Hiroshima University, School of Medicine, 1-2-3, Kasumi, Minami-ku, 734, Hiroshima, Japan aut NATIONALLICENCE 700 1- Hide M. Department of Dermatology, Hiroshima University, School of Medicine, 1-2-3, Kasumi, Minami-ku, 734, Hiroshima, Japan aut NATIONALLICENCE 700 1- Nakamura K. Department of Dermatology, Hiroshima University, School of Medicine, 1-2-3, Kasumi, Minami-ku, 734, Hiroshima, Japan aut NATIONALLICENCE 700 1- Yamamoto S. Department of Dermatology, Hiroshima University, School of Medicine, 1-2-3, Kasumi, Minami-ku, 734, Hiroshima, Japan aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/2(1990-01-01), 115-119 0340-3696 282:2<115 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer