caa a22 4500 465773982 CHVBK 20180323111936.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00493464 doi (NATIONALLICENCE)springer-10.1007/BF00493464 Stratum corneum antibodies detected by hemagglutination are not directed against keratin intermediate filaments [Elektronische Daten] [S. Qutaishat, V. Kumar, E. Beutner, S. Jabłońska] Summary: Autoantibodies to stratum corneum (SC) occur in virtually all normal adult human sera. These antibodies may be directed against various antigens of the SC. They have been detected by indirect immunofluorescence, passive hemagglutination (HA), immune adherence, and most recently by enzyme immunoassay and immunoblot methods. The purpose of our study was to examine whether antibodies to SC antigens as detected by passive HA are similar to the keratin intermediate filament (KIF) reactive antibodies. SC antigen preparation was prepared from psoriatic scales by the trypsin-phenol-water (TPW) extraction method. KIFs were prepared by 8 M urea extraction of normal callus or psoriatic scales. The anti-SC antibody titers of normal human sera were determined by passive HA before and after absorption with TPW-SC antigen preparation and upon absorption with KIFs. Similarly, titers of anti-KIF antibodies were determined on absorbed and unabsorbed sera by immunoblot assay. The results of this study indicate that the absorption of the sera with KIFs did not affect the titer of antibodies to TPW-extractable SC antigens whereas the titer of KIF antibodies dropped. KIF-reactive antibodies, on the other hand, were not affected by absorption with TPW-SC antigen, whereas the latter absorbed out the corresponding reactive antibodies. These results indicate that antibodies directed against SC antigen are different from the KIF-reactive antibodies. Springer-Verlag, 1990 Stratum corneum nationallicence Keratin intermediate filament nationallicence Autoantibodies nationallicence Qutaishat S. Department of Microbiology, The Ernest Witebsky Center for Immunology, 14214, Buffalo, NY, USA aut Kumar V. Department of Microbiology, The Ernest Witebsky Center for Immunology, 14214, Buffalo, NY, USA aut Beutner E. Department of Microbiology, The Ernest Witebsky Center for Immunology, 14214, Buffalo, NY, USA aut Jabłońska S. Department of Dermatology, Warsaw Academy of Medicine, Warsaw, Poland aut Archives of Dermatological Research Springer-Verlag 282/2(1990-01-01), 89-92 0340-3696 282:2<89 1990 282 403 https://doi.org/10.1007/BF00493464 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00493464 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Qutaishat S. Department of Microbiology, The Ernest Witebsky Center for Immunology, 14214, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Kumar V. Department of Microbiology, The Ernest Witebsky Center for Immunology, 14214, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Beutner E. Department of Microbiology, The Ernest Witebsky Center for Immunology, 14214, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Jabłońska S. Department of Dermatology, Warsaw Academy of Medicine, Warsaw, Poland aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/2(1990-01-01), 89-92 0340-3696 282:2<89 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer