caa a22 4500 465774016 CHVBK 20180323111936.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00493463 doi (NATIONALLICENCE)springer-10.1007/BF00493463 Immunoblot analyses of Brazilian pemphigus foliaceus antigen using different antigen sources [Elektronische Daten] [M. Ogawa, T. Hashimoto, A. Konohana, R. Castro, T. Nishikawa] Summary: We investigated the Brazilian pemphigus foliaceus (BPf) antigen applying the immunoblotting method to two different antigen sources using 27 patients' sera. Twelve BPf sera reacted specifically with a 150 kD protein in extract of dispase separated human epidermis, while 18 sera yielded a similar protein band in bovine muzzle desmosomal preparation. The diversity of staining intensities between the two samples suggested the heterogeneity of BPf antigens in terms of epitopes. Japanese sporadic pemphigus foliaceus (Pf) sera showed similar results but Japanese pemphigus vulgaris (Pv) sera recognized different antigens of 130 kD or 135 kD, suggesting that BPf is similar to Japanese Pf but is distinct from Pv in respect to the antigenic substance. Furthermore, the present study showed that immunoblot analysis using different antigen sources should be a valuable tool to determine clinical types of pemphigus. Springer-Verlag, 1990 Autoantibody nationallicence Autoantigen nationallicence Brazilian pemphigus foliaceus nationallicence Western blotting nationallicence Ogawa M. Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, 160, Tokyo, Japan aut Hashimoto T. Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, 160, Tokyo, Japan aut Konohana A. Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, 160, Tokyo, Japan aut Castro R. Department of Dermatology, Paulista College of Medicine, Sao Paulo, Brazil aut Nishikawa T. Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, 160, Tokyo, Japan aut Archives of Dermatological Research Springer-Verlag 282/2(1990-01-01), 84-88 0340-3696 282:2<84 1990 282 403 https://doi.org/10.1007/BF00493463 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00493463 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ogawa M. Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, 160, Tokyo, Japan aut NATIONALLICENCE 700 1- Hashimoto T. Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, 160, Tokyo, Japan aut NATIONALLICENCE 700 1- Konohana A. Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, 160, Tokyo, Japan aut NATIONALLICENCE 700 1- Castro R. Department of Dermatology, Paulista College of Medicine, Sao Paulo, Brazil aut NATIONALLICENCE 700 1- Nishikawa T. Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, 160, Tokyo, Japan aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/2(1990-01-01), 84-88 0340-3696 282:2<84 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer