caa a22 4500 465774032 CHVBK 20180323111937.0 cr unu---uuuuu 170327e19900101xx s 000 0 eng 10.1007/BF00493473 doi (NATIONALLICENCE)springer-10.1007/BF00493473 Polyprenoic acid, E-5166, is effective in inhibiting the proliferation of keratinocytes in vitro [Elektronische Daten] [M. Koji, T. Kanzaki, A. Takashima] Summary: E-5166 is a newly synthesized polyprenoic acid that has been reported to control epithelial differentiation and to have antiproliferative effects on various tumor cells in vitro. This study examined the effects of E-5166 on the proliferation of keratinocytes. Three kinds of keratinocytes were used: normal human keratinocytes, a human trichilemmoma-derived K-TL-1 cell line, and a Pam 212 cell line. Cell proliferation was measured by 3H-thymidine incorporation and also by determining cell numbers. E-5166 was found to have significant antiproliferative effects on each of the cell lines studied. When Pam 212 cells were cultured in the presence of E-5166, cell proliferation was inhibited in a dose-dependent fashion. The inhibitory effect appeared to be reversible, since removal of E-5166 from the culture medium resulted in a subsequent return of cell proliferation. For normal human keratinocytes and K-TL-1 cells, E-5166 inhibited 3H-thymidine incorporation in a dose-dependent fashion. Furthermore, E-5166 showed significantly stronger antiproliferative capacity than Ro 10-9359, one of the aromatic retinoids, on Pam 212 cells and normal human keratinocytes. Springer-Verlag, 1990 Retinoic acid nationallicence Keratinocytes nationallicence Proliferating E-5166 nationallicence Koji M. Department of Dermatology, Nagoya City University Medical School, Mizuho-cho, Mizuho-ku 467, Nagoya, Japan aut Kanzaki T. Department of Dermatology, Nagoya City University Medical School, Mizuho-cho, Mizuho-ku 467, Nagoya, Japan aut Takashima A. Department of Dermatology, Nagoya City University Medical School, Mizuho-cho, Mizuho-ku 467, Nagoya, Japan aut Archives of Dermatological Research Springer-Verlag 282/2(1990-01-01), 135-138 0340-3696 282:2<135 1990 282 403 https://doi.org/10.1007/BF00493473 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00493473 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koji M. Department of Dermatology, Nagoya City University Medical School, Mizuho-cho, Mizuho-ku 467, Nagoya, Japan aut NATIONALLICENCE 700 1- Kanzaki T. Department of Dermatology, Nagoya City University Medical School, Mizuho-cho, Mizuho-ku 467, Nagoya, Japan aut NATIONALLICENCE 700 1- Takashima A. Department of Dermatology, Nagoya City University Medical School, Mizuho-cho, Mizuho-ku 467, Nagoya, Japan aut NATIONALLICENCE 773 0- Archives of Dermatological Research Springer-Verlag 282/2(1990-01-01), 135-138 0340-3696 282:2<135 1990 282 403 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer